opuD

168

glycine betaine and arsenobetaine transporter

Locus: BSU_30070

Molecular weight: 55.95 kDa

Isoelectric point: 9.51

Protein length: 512 aa

Gene length: 1539 bp

Function: compatible solute transport

Product: glycine betaine transporter

Essential: no

Synonyms: opuD, ytfQ

Genomic Context

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List of homologs in different organisms, belongs to COG1292 (Galperin et al., 2021)

Gene

Coordinates: 3,076,818  3,078,356

The protein

Catalyzed reaction/ biological activity

• uptake of glycine betaine and arsenobetaine PubMed

Protein family

• BCCT transporter (TC 2.A.15) family (single member, according to UniProt)

Structure

• 4AIN (PDB) (BetP from Corynebacterium glutamicum, 44% identity) PubMed
• P54417 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ytfP-opuD

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: opuD

  Description

  • PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon

Biological materials

Mutant

• MGNA-A815 (opuD::erm), available at the NBRP B. subtilis, Japan
• 1A1052 ( opuD::kan), PubMed, available at BGSC
• 1A1053 ( opuD::kan), PubMed, available at BGSC
• 1A1054 ( opuD::kan), PubMed, available at BGSC
• 1A1055 ( opuD::kan), PubMed, available at BGSC
• BKE30070 (opuD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTCACCCTCTAATTT,  downstream forward: _UP4_TAACAAAAAAGATCTTTCCG
• BKK30070 (opuD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCTCACCCTCTAATTT,  downstream forward: _UP4_TAACAAAAAAGATCTTTCCG

Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

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Original Publications

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