YlmB

• Description: N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase

Gene name	ylmB
Synonyms
Essential	no
Product	N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine deformylase
Function	thiamine salvage
Gene expression levels in SubtiExpress: ylmB
Metabolic function and regulation of this protein in SubtiPathways: Thiamin
MW, pI	47 kDa, 4.981
Gene length, protein length	1278 bp, 426 aa
Immediate neighbours	ylmA, ylmC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Thi-box

The gene

Basic information

• Locus tag: BSU15350

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: N-formyl-4-amino-5-aminomethyl-2-methylpyrimidine + H2O = formate + 4-amino-5-aminomethyl-2-methylpyrimidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s): YodQ

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):Binds 2 zinc or cobalt ions per subunit (according to Swiss-Prot)

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O31724

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: ylmB PubMed

• Sigma factor:

• Regulation:
  • repressed by thiamine (Thi-box) PubMed

• Regulatory mechanism:
  • Thi-box: RNA switch PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Amy Haas Jenkins, Ghislain Schyns, Sébastien Potot, Guangxing Sun, Tadhg P Begley
A new thiamin salvage pathway.
Nat Chem Biol: 2007, 3(8);492-7
[PubMed:17618314] [WorldCat.org] [DOI] (P p)

Dmitry A Rodionov, Alexey G Vitreschak, Andrey A Mironov, Mikhail S Gelfand
Comparative genomics of thiamin biosynthesis in procaryotes. New genes and regulatory mechanisms.
J Biol Chem: 2002, 277(50);48949-59
[PubMed:12376536] [WorldCat.org] [DOI] (P p)