YetO

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Gene name yetO
Synonyms yfnJ , cypD
Essential no
Product NADPH-cytochrome P450 reductase
Function fatty acid metabolism
Gene expression levels in SubtiExpress: yetO
MW, pI 119 kDa, 5.732
Gene length, protein length 3183 bp, 1061 aa
Immediate neighbours yetN, yfnI
Sequences Protein DNA DNA_with_flanks
Genetic context
YetO context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YetO expression.png

























Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU07250

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • oxidation of even- and odd-chain saturated and unsaturated fatty acids to the respective omega-3, omega-2 and omega-1 hydroxylated fatty acids PubMed
  • Protein family:
  • Paralogous protein(s): YrhJ

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2X7Y (from Bacillus megaterium; 66% identity, 87% similarity)
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Irene Axarli, Ariadne Prigipaki, Nikolaos E Labrou
Cytochrome P450 102A2 Catalyzes Efficient Oxidation of Sodium Dodecyl Sulphate: A Molecular Tool for Remediation.
Enzyme Res: 2010, 2010;125429
[PubMed:21048857] [WorldCat.org] [DOI] (I e)

Sabine Eiben, Leonard Kaysser, Steffen Maurer, Katja Kühnel, Vlada B Urlacher, Rolf D Schmid
Preparative use of isolated CYP102 monooxygenases -- a critical appraisal.
J Biotechnol: 2006, 124(4);662-9
[PubMed:16716428] [WorldCat.org] [DOI] (P p)

Irene Axarli, Ariadne Prigipaki, Nikolaos E Labrou
Engineering the substrate specificity of cytochrome P450 CYP102A2 by directed evolution: production of an efficient enzyme for bioconversion of fine chemicals.
Biomol Eng: 2005, 22(1-3);81-8
[PubMed:15857787] [WorldCat.org] [DOI] (P p)

M Budde, S C Maurer, R D Schmid, V B Urlacher
Cloning, expression and characterisation of CYP102A2, a self-sufficient P450 monooxygenase from Bacillus subtilis.
Appl Microbiol Biotechnol: 2004, 66(2);180-6
[PubMed:15375636] [WorldCat.org] [DOI] (P p)

Mattias C U Gustafsson, Olivier Roitel, Ker R Marshall, Michael A Noble, Stephen K Chapman, Antonio Pessegueiro, Armand J Fulco, Myles R Cheesman, Claes von Wachenfeldt, Andrew W Munro
Expression, purification, and characterization of Bacillus subtilis cytochromes P450 CYP102A2 and CYP102A3: flavocytochrome homologues of P450 BM3 from Bacillus megaterium.
Biochemistry: 2004, 43(18);5474-87
[PubMed:15122913] [WorldCat.org] [DOI] (P p)

M C Gustafsson, C N Palmer, C R Wolf, C von Wachenfeldt
Fatty-acid-displaced transcriptional repressor, a conserved regulator of cytochrome P450 102 transcription in Bacillus species.
Arch Microbiol: 2001, 176(6);459-64
[PubMed:11734890] [WorldCat.org] [DOI] (P p)