PdaA

• Description: polysaccharide deacetylase, required for germination
  
  

Gene name	pdaA
Synonyms	yfjS
Essential	no
Product	polysaccharide deacetylase
Function	germination
Gene expression levels in SubtiExpress: pdaA
MW, pI	29 kDa, 7.318
Gene length, protein length	789 bp, 263 aa
Immediate neighbours	yfjT, yfjR
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

germination

This gene is a member of the following regulons

SigG regulon

The gene

Basic information

• Locus tag: BSU07980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: polysaccharide deacetylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1W17

• UniProt: O34928

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: pdaA PubMed

• Expression browser: pdaA PubMed

• Sigma factor: SigG PubMed

• Regulation: expressed late during sporulation in the forespore (SigG) PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Toshihiko Kitajima, Junichi Sekiguchi
A polysaccharide deacetylase homologue, PdaA, in Bacillus subtilis acts as an N-acetylmuramic acid deacetylase in vitro.
J Bacteriol: 2005, 187(4);1287-92
[PubMed:15687192] [WorldCat.org] [DOI] (P p)

David E Blair, Daan M F van Aalten
Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine.
FEBS Lett: 2004, 570(1-3);13-9
[PubMed:15251431] [WorldCat.org] [DOI] (P p)

Meghan E Gilmore, Dabanjan Bandyopadhyay, Amanda M Dean, Sarah D Linnstaedt, David L Popham
Production of muramic delta-lactam in Bacillus subtilis spore peptidoglycan.
J Bacteriol: 2004, 186(1);80-9
[PubMed:14679227] [WorldCat.org] [DOI] (P p)

Tatsuya Fukushima, Hiroki Yamamoto, Abdelmadjid Atrih, Simon J Foster, Junichi Sekiguchi
A polysaccharide deacetylase gene (pdaA) is required for germination and for production of muramic delta-lactam residues in the spore cortex of Bacillus subtilis.
J Bacteriol: 2002, 184(21);6007-15
[PubMed:12374835] [WorldCat.org] [DOI] (P p)