yfjR

168

similar to 3-hydroxyisobutyrate dehydrogenase

Locus: BSU_07990

Molecular weight: 27.72 kDa

Isoelectric point: 4.78

Protein length: 286 aa

Gene length: 861 bp

Function: unknown

Product: 3-hydroxyisobutyrate dehydrogenase

Essential: no

Synonyms: yfjR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2084 (Galperin et al., 2021)

Gene

Coordinates: 870,388  871,248

The protein

Protein family

• HIBADH-related family (with YkwC, according to UniProt)

Structure

• 4GBJ (PDB) (from Dyadobacter Fermentans, 41% identity)
• O34969 (AlphaFold)

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Paralogous protein(s)

• YkwC, (32%)

Expression and Regulation

Operons

• Genes: yfjR

  Description: PubMed

  Regulatory mechanism

  • YclJ: activation, PubMed, in yclJ regulon

Biological materials

Mutant

• MGNA-C350 (yfjR::erm), available at the NBRP B. subtilis, Japan
• BKE07990 (yfjR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCTAATCACTCCCTGAA,  downstream forward: _UP4_TAAAGAAAAGCCTCTCCGTT
• BKK07990 (yfjR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATCTAATCACTCCCTGAA,  downstream forward: _UP4_TAAAGAAAAGCCTCTCCGTT

References

Loading