Hag

• Description: flagellin protein, about 20,000 subunits make up one flagellum
  
  

Gene name	hag
Synonyms
Essential	no
Product	flagellin protein
Function	motility and chemotaxis
Gene expression levels in SubtiExpress: hag
Interactions involving this protein in SubtInteract: Hag
MW, pI	32 kDa, 4.782
Gene length, protein length	912 bp, 304 aa
Immediate neighbours	yvyC, csrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, CsrA regulon, ScoC regulon, SigD regulon

The gene

Basic information

• Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial flagellin family (according to Swiss-Prot)

• Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FlhA-Hag PubMed
  • FlhA-(FliS-Hag) PubMed
  • FliS-Hag PubMed
  • FliW-Hag PubMed

• Localization:
  • extracellular (no signal peptide), major component of the secretome PubMed
  • membrane PubMed

Database entries

• Structure:

• UniProt: P02968

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hag PubMed

• Expression browser: hag PubMed

• Sigma factor: SigD PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • CsrA: translation inhibition PubMed
  • ScoC: transcription repression PubMed

• Additional information:
  • the hag gene is strongly overexpressed in a ymdB mutant (loss of bistable gene expression) PubMed

Biological materials

• Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab
  • 1A915 ( hag::cat), PubMed, available at BGSC
  • 1A783 ( hag::erm), PubMed, available at BGSC
  • 1A842 ( hag::kan), PubMed, available at BGSC

• Expression vector:
  • expression in B. subtilis, in pBQ200: pGP1089, available in Stülke lab

• lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab

• GFP fusion: BP494 (bglS:: (hag-promoter-cfp-aphA3)), BP496 (amyE:: (hag-promoter-iyfp-cat)), available in Stülke lab

• two-hybrid system:

• Antibody:

References

Original Publications

Additional publications: PubMed

Sampriti Mukherjee, Paul Babitzke, Daniel B Kearns
FliW and FliS function independently to control cytoplasmic flagellin levels in Bacillus subtilis.
J Bacteriol: 2013, 195(2);297-306
[PubMed:23144244] [WorldCat.org] [DOI] (I p)

Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J  
A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects
Flagellin Expression and Biofilm Formation. 
J Bacteriol.: 2011, 193(21):5997-6007. 
PubMed:21856853