FolE2

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  • Description: GTP cyclohydrolase IB, replaces FolE under conditions of zinc starvation

Gene name folE2
Synonyms yciA, folEB
Essential no
Product zinc-independent GTP cyclohydrolase IB
Function biosynthesis of folate
Gene expression levels in SubtiExpress: folE2
MW, pI 34 kDa, 7.831
Gene length, protein length 915 bp, 305 aa
Immediate neighbours nasA, yciB
Sequences Protein DNA Advanced_DNA
Genetic context
YciA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FolE2 expression.png
























Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

Zur regulon

The gene

Basic information

  • Locus tag: BSU03340

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: GTP + H2O = formate + 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)-dihydropteridine triphosphate (according to Swiss-Prot)
  • Protein family: GTP cyclohydrolase IV family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s): activated by a variety of divalent cations PubMed
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
    • Zur: transcription repression in the presence of zinc PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Banumathi Sankaran, Shilah A Bonnett, Kinjal Shah, Scott Gabriel, Robert Reddy, Paul Schimmel, Dmitry A Rodionov, Valérie de Crécy-Lagard, John D Helmann, Dirk Iwata-Reuyl, Manal A Swairjo
Zinc-independent folate biosynthesis: genetic, biochemical, and structural investigations reveal new metal dependence for GTP cyclohydrolase IB.
J Bacteriol: 2009, 191(22);6936-49
[PubMed:19767425] [WorldCat.org] [DOI] (I p)

Basma El Yacoubi, Shilah Bonnett, Jessica N Anderson, Manal A Swairjo, Dirk Iwata-Reuyl, Valérie de Crécy-Lagard
Discovery of a new prokaryotic type I GTP cyclohydrolase family.
J Biol Chem: 2006, 281(49);37586-93
[PubMed:17032654] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

A Gaballa, J D Helmann
Identification of a zinc-specific metalloregulatory protein, Zur, controlling zinc transport operons in Bacillus subtilis.
J Bacteriol: 1998, 180(22);5815-21
[PubMed:9811636] [WorldCat.org] [DOI] (P p)