AnsR

• Description: transcription repressor of the ansA-ansB operon
  
  

Gene name	ansR
Synonyms
Essential	no
Product	transcriptional regulator (Xre family)
Function	negative regulation of the ansA-ansB operon
Gene expression levels in SubtiExpress: ansR
Metabolic function and regulation of this protein in SubtiPathways: Asp, Asn
MW, pI	13 kDa, 4.807
Gene length, protein length	348 bp, 116 aa
Immediate neighbours	ansA, yqxK
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of amino acids, transcription factors and their control

This gene is a member of the following regulons

AnsR regulon

The AnsR regulon:

• ** ansA-ansB-mleN-mleA, ansR

The gene

Basic information

• Locus tag: BSU23590

Phenotypes of a mutant

• the ansR mutation allows glutamate utilization of a rocG gudB mutant PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: Q07683

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: ansR PubMed

• Expression browser: ansR PubMed

• Sigma factor:

• Regulation:
  • induced in the presence of asparagine (AnsR) PubMed

• Regulatory mechanism:
  • AnsR: negative autoregulation PubMed

• Additional information:

Biological materials

• Mutant: GP1152 (del tet) available in the Stülke lab

• Expression vector:
  • expression of native ansR in B. subtilis: pGP873 (in pBQ200), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lope A Flórez, Katrin Gunka, Rafael Polanía, Stefan Tholen, Jörg Stülke
SPABBATS: A pathway-discovery method based on Boolean satisfiability that facilitates the characterization of suppressor mutants.
BMC Syst Biol: 2011, 5;5
[PubMed:21219666] [WorldCat.org] [DOI] (I e)

Susan H Fisher, Lewis V Wray
Bacillus subtilis 168 contains two differentially regulated genes encoding L-asparaginase.
J Bacteriol: 2002, 184(8);2148-54
[PubMed:11914346] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning and nucleotide sequence of the Bacillus subtilis ansR gene, which encodes a repressor of the ans operon coding for L-asparaginase and L-aspartase.
J Bacteriol: 1993, 175(9);2501-6
[PubMed:8478318] [WorldCat.org] [DOI] (P p)