IolE

• Description: 2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
  
  

Gene name	iolE
Synonyms	yxdE
Essential	no
Product	2-keto-myo-inositol dehydratase, dehydration of 2-keto-myo-inositol (2nd reaction)
Function	myo-inositol catabolism
Gene expression levels in SubtiExpress: iolE
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	33 kDa, 5.209
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	iolF, iolD
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

CcpA regulon, IolR regulon

The gene

Basic information

• Locus tag: BSU39720

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2,4,6/3,5-pentahydroxycyclohexanone = 3,5/4-trihydroxycyclohexa-1,2-dione + H₂O (according to Swiss-Prot)

• Protein family: iolE/mocC family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3CNY (from Lactobacillus plantarum wcfs1 mutant, 63% identity, 78% similarity)

• UniProt: P42416

• KEGG entry: [3]

• E.C. number: 4.2.1.44

Expression and regulation

• Operon: iolA-iolB-iolC-iolD-iolE-iolF-iolG-iolH-iolI-iolJ PubMed

• Expression browser: iolE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by inositol (IolR) PubMed

• Regulatory mechanism:
  • IolR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

• Yasutaro Fujita, University of Fukuyama, Japan
• Ken-ichi Yoshida, Kobe University, Japan