Dra

• Description: deoxyribose-phosphate aldolase
  
  

Gene name	dra
Synonyms	deoC
Essential	no
Product	deoxyribose-phosphate aldolase
Function	utilization of nucleotides as carbon source
Gene expression levels in SubtiExpress: dra
Metabolic function and regulation of this protein in SubtiPathways: Nucleoside catabolism, Nucleotides (regulation)
MW, pI	22 kDa, 4.926
Gene length, protein length	633 bp, 211 aa
Immediate neighbours	nupC, deoR
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed 500px

Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

CcpA regulon, DeoR regulon

The gene

Basic information

• Locus tag: BSU39420

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde (according to Swiss-Prot)

• Protein family: DeoC type 1 subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1MZH (from Aquifex aeolicus, 42% identity, 62% similarity)

• UniProt: P39121

• KEGG entry: [3]

• E.C. number: 4.1.2.4

Expression and regulation

• Operon: dra-nupC-pdp PubMed

• Expression browser: dra PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • induced by deoxynucleotides (DeoR) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • DeoR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xianmin Zeng, Anne Galinier, Hans H Saxild
Catabolite repression of dra-nupC-pdp operon expression in Bacillus subtilis.
Microbiology (Reading): 2000, 146 ( Pt 11);2901-2908
[PubMed:11065368] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)

X Zeng, H H Saxild
Identification and characterization of a DeoR-specific operator sequence essential for induction of dra-nupC-pdp operon expression in Bacillus subtilis.
J Bacteriol: 1999, 181(6);1719-27
[PubMed:10074062] [WorldCat.org] [DOI] (P p)

H H Saxild, L N Andersen, K Hammer
Dra-nupC-pdp operon of Bacillus subtilis: nucleotide sequence, induction by deoxyribonucleosides, and transcriptional regulation by the deoR-encoded DeoR repressor protein.
J Bacteriol: 1996, 178(2);424-34
[PubMed:8550462] [WorldCat.org] [DOI] (P p)