AlaS

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  • Description: alanine-tRNA synthetase

Gene name alaS
Synonyms
Essential yes PubMed
Product alanine-tRNA synthetase
Function translation
Gene expression levels in SubtiExpress: alaS
Metabolic function and regulation of this protein in SubtiPathways:
tRNA charging
MW, pI 97 kDa, 4.922
Gene length, protein length 2634 bp, 878 aa
Immediate neighbours yrzL, yrrI
Sequences Protein DNA Advanced_DNA
Genetic context
AlaS context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AlaS expression.png




























Categories containing this gene/protein

translation, essential genes

This gene is a member of the following regulons

T-box

The gene

Basic information

  • Locus tag: BSU27410

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala) (according to Swiss-Prot)
  • Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1YFS (enzyme from Aquifex aeolicus) PubMed, 3HXU (enzyme from E. coli with alanine) PubMed
  • KEGG entry: [2]

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Min Guo, Yeeting E Chong, Ryan Shapiro, Kirk Beebe, Xiang-Lei Yang, Paul Schimmel
Paradox of mistranslation of serine for alanine caused by AlaRS recognition dilemma.
Nature: 2009, 462(7274);808-12
[PubMed:20010690] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Manal A Swairjo, Paul R Schimmel
Breaking sieve for steric exclusion of a noncognate amino acid from active site of a tRNA synthetase.
Proc Natl Acad Sci U S A: 2005, 102(4);988-93
[PubMed:15657145] [WorldCat.org] [DOI] (P p)