reoM

168

effector protein of MurAA degradation by ClpC-ClpP

Locus: BSU_27400

Molecular weight: 10.10 kDa

Isoelectric point: 4.73

Protein length: 88 aa

Gene length: 267 bp

Function: control of peptidoglycan biosynthesis

Product: regulator of MurAA degradation

Essential: no

Synonyms: reoM, yrzL

Genomic Context

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List of homologs in different organisms, belongs to COG4472 (Galperin et al., 2021)

Gene

Coordinates: 2,797,823 → 2,798,089

Phenotypes of a mutant

• accumulation of MurAA due to reduced degradation by ClpC-ClpP PubMed

The protein

Protein family

• UPF0297 family (single member, according to UniProt)

Structure

• 5US5 (PDB) (from Enterococcus faecalis, 66% identity) PubMed
• 6TIF (PDB) (from Listeria monocytogenes, 68% identity) PubMed
• Q7WY61 (AlphaFold)

Expression and Regulation

Operons

• Genes: reoM-yrrK-yrzB

  Description: PubMed

• Genes: alaS-reoM-yrrK-yrzB

  Description: PubMed

  Regulation

  • induced by alanine limitation ([regulon|T-box|]) PubMed

  Regulatory mechanism

  • T-box: RNA switch, [pubmed|], in T-box

Biological materials

Mutant

• BKE27400 (ΔreoM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCGTTTTGCACCTCTTTC,  downstream forward: _UP4_CAGCATAAAGAGGCGTAAAT
• BKK27400 (ΔreoM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACCGTTTTGCACCTCTTTC,  downstream forward: _UP4_CAGCATAAAGAGGCGTAAAT

References

Research papers

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