MmgE

• Description: putative methyl-cis-aconitase
  
  

Gene name	mmgE
Synonyms	yqiP, prpD
Essential	no
Product	unknown
Function	mother cell metabolism
Gene expression levels in SubtiExpress: mmgE
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	52 kDa, 6.111
Gene length, protein length	1416 bp, 472 aa
Immediate neighbours	yqiQ, mmgD
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, poorly characterized/ putative enzymes

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU24130

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate = (Z)-but-2-ene-1,2,3-tricarboxylate + H₂O (according to Swiss-Prot)

• Protein family: prpD family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): contains an iron-sulfur cluster

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45859

• KEGG entry: [3]

• E.C. number: 4.2.1.79

Expression and regulation

• Operon: mmgA-mmgB-mmgC-mmgD-mmgE-yqiQ PubMed

• Expression browser: mmgE PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

E M Bryan, B W Beall, C P Moran
A sigma E dependent operon subject to catabolite repression during sporulation in Bacillus subtilis.
J Bacteriol: 1996, 178(16);4778-86
[PubMed:8759838] [WorldCat.org] [DOI] (P p)