ComGA

• Description: late competence protein, traffic ATPase, binds and transports transforming DNA
  
  

Gene name	comGA
Synonyms
Essential	no
Product	traffic ATPase
Function	genetic competence
Gene expression levels in SubtiExpress: comGA
Interactions involving this protein in SubtInteract: ComGA
Metabolic function and regulation of this protein in SubtiPathways: Protein secretion
MW, pI	40 kDa, 8.893
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	comGB, corA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU24730

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds and transports transforming DNA

• Protein family: GSP E family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGA-ComGB PubMed
  • ComGA-ComFA PubMed
  • ComEC-ComGA PubMed
  • Maf-ComGA PubMed

• Localization:
  • peripheral membrane protein PubMed
  • localizes to cell poles in competent cells PubMed
  • localizes to one cell pole PubMed

Database entries

• Structure:

• UniProt: P25953

• KEGG entry: [3]

• E.C. number:

Additional information

• ComGA stability depends on Maf PubMed

Expression and regulation

• Operon: comGA-comGB-comGC-comGD-comGE-comGF-comGG-yqzE PubMed

• Expression browser: comGA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)