YjiB

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Gene name yjiB
Synonyms
Essential no
Product monooxygenase CYP109B1
Function oxidation of fatty acids
Gene expression levels in SubtiExpress: yjiB
Interactions involving this protein in SubtInteract: YjiB
MW, pI 44 kDa, 5.379
Gene length, protein length 1188 bp, 396 aa
Immediate neighbours yjiA, yjiC
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YjiB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YjiB expression.png
























Categories containing this gene/protein

electron transport/ other, lipid metabolism/ other

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU12210

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • oxidation of saturated fatty acids (conversion up to 99%) and their methyl and ethyl esters (conversion up to 80%) at subterminal positions with a preference for the carbon atoms C11 and C12 counted from the carboxyl group PubMed
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Operon:
  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Marco Girhard, Tobias Klaus, Yogan Khatri, Rita Bernhardt, Vlada B Urlacher
Characterization of the versatile monooxygenase CYP109B1 from Bacillus subtilis.
Appl Microbiol Biotechnol: 2010, 87(2);595-607
[PubMed:20186410] [WorldCat.org] [DOI] (I p)

Marco Girhard, Kazuhiro Machida, Masashi Itoh, Rolf D Schmid, Akira Arisawa, Vlada B Urlacher
Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system.
Microb Cell Fact: 2009, 8;36
[PubMed:19591681] [WorldCat.org] [DOI] (I e)