RapF

• Description: response regulator aspartate phosphatase, anti-activator protein, controls ComA activity
  
  

Gene name	rapF
Synonyms	ywhJ
Essential	no
Product	response regulator aspartate phosphatase
Function	control of ComA activity
Interactions involving this protein in SubtInteract: RapF
MW, pI	45 kDa, 5.18
Gene length, protein length	1143 bp, 381 aa
Immediate neighbours	ywhK, phrF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence, protein modification, transcription factors and their control, quorum sensing

This gene is a member of the following regulons

CcpA regulon, ComA regulon

The gene

Basic information

• Locus tag: BSU37460

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: control of ComA activity PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: binding of PhrF to RapF results in a conformational change of RapF and prevents it from binding ComA PubMed

• Interactions:
  • RapF-ComA PubMed
  • RapF-PhrF PubMed

• Localization:

Database entries

• Structure:
  • 3ULQ (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed

• UniProt: P71002

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapF-phrF PubMed

• Expression browser: rapF PubMed

• Sigma factor:

• Regulation: repressed by glucose (5-fold) (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • ComA: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: