ThiE

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  • Description: thiamine-phosphate diphosphorylase

Gene name thiE
Synonyms thiC, ipa-26d, ywbK
Essential no
Product thiamine-phosphate diphosphorylase
Function biosynthesis of thiamine
by 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate
to yield thiamine phosphate
Metabolic function and regulation of this protein in SubtiPathways:
Thiamin
MW, pI 23 kDa, 5.058
Gene length, protein length 666 bp, 222 aa
Immediate neighbours ywbL, thiM
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
ThiE context.gif
This image was kindly provided by SubtiList









Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU38290

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate (according to Swiss-Prot)
  • Protein family: TMP-PPase family (according to Swiss-Prot)
  • Paralogous protein(s): TenI

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Christopher T Jurgenson, Tadhg P Begley, Steven E Ealick
The structural and biochemical foundations of thiamin biosynthesis.
Annu Rev Biochem: 2009, 78;569-603
[PubMed:19348578] [WorldCat.org] [DOI] (I p)

Original publications