Hag

• Description: flagellin protein
  
  

Gene name	hag
Synonyms
Essential	no
Product	flagellin protein
Function	motility and chemotaxis
Interactions involving this protein in SubtInteract: Hag
MW, pI	32 kDa, 4.782
Gene length, protein length	912 bp, 304 aa
Immediate neighbours	yvyC, csrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

motility and chemotaxis

This gene is a member of the following regulons

CodY regulon, CsrA regulon, ScoC regulon, SigD regulon

The gene

Basic information

• Locus tag: BSU35360

Phenotypes of a mutant

No swarming motility on B medium. PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial flagellin family (according to Swiss-Prot)

• Paralogous protein(s): YvzB (C-terminal domain of Hag)

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • FlhA-Hag PubMed
  • FlhA-(FliS-Hag) PubMed
  • FliS-Hag PubMed
  • FliW-Hag PubMed

• Localization:
  • extracellular (no signal peptide), major component of the secretome PubMed
  • membrane PubMed

Database entries

• Structure:

• UniProt: P02968

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hag PubMed

• Sigma factor: SigD PubMed

• Regulation:
  • repressed during growth in the presence of branched chain amino acids (CodY) PubMed

• Regulatory mechanism:
  • CodY: transcription repression PubMed
  • CsrA: translation inhibition PubMed
  • ScoC: transcription repression PubMed

• Additional information:
  • the hag gene is strongly overexpressed in a ymdB mutant (loss of bistable gene expression) PubMed

Biological materials

• Mutant: GP901 (aphA3), GP902 (tet), both available in Stülke lab

• Expression vector:

• lacZ fusion: pGP1035 (in pAC6), pGP755 (in pAC7), there is also a series of promoter deletion variants in pAC6 and pAC7, all available in Stülke lab

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J  
A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects
Flagellin Expression and Biofilm Formation. 
J Bacteriol.: 2011, 193(21):5997-6007. 
PubMed:21856853

Sampriti Mukherjee, Helen Yakhnin, Dave Kysela, Josh Sokoloski, Paul Babitzke, Daniel B Kearns
CsrA-FliW interaction governs flagellin homeostasis and a checkpoint on flagellar morphogenesis in Bacillus subtilis.
Mol Microbiol: 2011, 82(2);447-61
[PubMed:21895793] [WorldCat.org] [DOI] (I p)

Kassem Hamze, Sabine Autret, Krzysztof Hinc, Soumaya Laalami, Daria Julkowska, Romain Briandet, Margareth Renault, Cédric Absalon, I Barry Holland, Harald Putzer, Simone J Séror
Single-cell analysis in situ in a Bacillus subtilis swarming community identifies distinct spatially separated subpopulations differentially expressing hag (flagellin), including specialized swarmers.
Microbiology (Reading): 2011, 157(Pt 9);2456-2469
[PubMed:21602220] [WorldCat.org] [DOI] (I p)

Gert Bange, Nico Kümmerer, Christoph Engel, Gunes Bozkurt, Klemens Wild, Irmgard Sinning
FlhA provides the adaptor for coordinated delivery of late flagella building blocks to the type III secretion system.
Proc Natl Acad Sci U S A: 2010, 107(25);11295-300
[PubMed:20534509] [WorldCat.org] [DOI] (I p)

Prashant Kodgire, K Krishnamurthy Rao
hag expression in Bacillus subtilis is both negatively and positively regulated by ScoC.
Microbiology (Reading): 2009, 155(Pt 1);142-149
[PubMed:19118355] [WorldCat.org] [DOI] (P p)

Helen Yakhnin, Pallavi Pandit, Tom J Petty, Carol S Baker, Tony Romeo, Paul Babitzke
CsrA of Bacillus subtilis regulates translation initiation of the gene encoding the flagellin protein (hag) by blocking ribosome binding.
Mol Microbiol: 2007, 64(6);1605-20
[PubMed:17555441] [WorldCat.org] [DOI] (P p)