PyrH

• Description: uridylate kinase
  
  

Gene name	pyrH
Synonyms	smbA
Essential	no
Product	uridylate kinase
Function	pyrimidine biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Nucleotides (regulation)
MW, pI	25 kDa, 5.101
Gene length, protein length	720 bp, 240 aa
Immediate neighbours	tsf, frr
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16510

Expression

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UMP = ADP + UDP (according to Swiss-Prot)

• Protein family: UMP kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2JJX (from Bacillus anthracis, 42% identity, 64% similarity) PubMed

• UniProt: O31749

• KEGG entry: [2]

• E.C. number: 2.7.4.22

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Cristina Gagyi, Nadia Bucurenci, Ovidiu Sîrbu, Gilles Labesse, Mihaela Ionescu, Augustin Ofiteru, Liliane Assairi, Stéphanie Landais, Antoine Danchin, Octavian Bârzu, Anne-Marie Gilles
UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity.
Eur J Biochem: 2003, 270(15);3196-204
[PubMed:12869195] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)