SpoVD

• Description: mother-cell specific penicillin-binding protein (spore cortex)
  
  

Gene name	spoVD
Synonyms
Essential	no
Product	penicillin-binding protein (spore cortex)
Function	spore morphogenesis
Interactions involving this protein in SubtInteract: SpoVD
MW, pI	71 kDa, 8.85
Gene length, protein length	1935 bp, 645 aa
Immediate neighbours	pbpB, murE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

cell wall synthesis, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU15170

Expression

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: protects SpoVE from proteolytic degradation PubMed

• Protein family:

• Paralogous protein(s): PbpB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: intramolecular disulfide bonds between two Cys residues are reduced by StoA, this is rquired for activity of SpoVD PubMed

• Interactions:
  • SpoVD-StoA PubMed
  • SpoVD-SpoVE PubMed

• Localization: intermembrane space that separates forespores from mother cells PubMed

Database entries

• Structure:

• UniProt: Q03524

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoVD PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE) PubMed

• Regulatory mechanism:

• • SpoIIID: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Patrick Eichenberger
The red-ox status of a penicillin-binding protein is an on/off switch for spore peptidoglycan synthesis in Bacillus subtilis.
Mol Microbiol: 2010, 75(1);10-2
[PubMed:19919674] [WorldCat.org] [DOI] (I p)

Original Publications

Allison Fay, Pablo Meyer, Jonathan Dworkin
Interactions between late-acting proteins required for peptidoglycan synthesis during sporulation.
J Mol Biol: 2010, 399(4);547-61
[PubMed:20417640] [WorldCat.org] [DOI] (I p)

Yiming Liu, Mirja Carlsson Möller, Lise Petersen, Christopher A G Söderberg, Lars Hederstedt
Penicillin-binding protein SpoVD disulphide is a target for StoA in Bacillus subtilis forespores.
Mol Microbiol: 2010, 75(1);46-60
[PubMed:19919673] [WorldCat.org] [DOI] (I p)

Dirk-Jan Scheffers
Dynamic localization of penicillin-binding proteins during spore development in Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 3);999-1012
[PubMed:15758244] [WorldCat.org] [DOI] (P p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

B Zhang, R A Daniel, J Errington, L Kroos
Bacillus subtilis SpoIIID protein binds to two sites in the spoVD promoter and represses transcription by sigmaE RNA polymerase.
J Bacteriol: 1997, 179(3);972-5
[PubMed:9006059] [WorldCat.org] [DOI] (P p)

R A Daniel, S Drake, C E Buchanan, R Scholle, J Errington
The Bacillus subtilis spoVD gene encodes a mother-cell-specific penicillin-binding protein required for spore morphogenesis.
J Mol Biol: 1994, 235(1);209-20
[PubMed:8289242] [WorldCat.org] [DOI] (P p)

R A Daniel, J Errington
DNA sequence of the murE-murD region of Bacillus subtilis 168.
J Gen Microbiol: 1993, 139(2);361-70
[PubMed:8436954] [WorldCat.org] [DOI] (P p)