NadB

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  • Description: L-aspartate oxidase

Gene name nadB
Synonyms
Essential no
Product L-aspartate oxidase
Function NAD biosynthesis
MW, pI 58 kDa, 6.445
Gene length, protein length 1593 bp, 531 aa
Immediate neighbours nadC, nifS
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
NadB context.gif
This image was kindly provided by SubtiList



Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

NadR regulon

The gene

Basic information

  • Locus tag: BSU27870

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate + O2 = iminosuccinate + H2O2 (according to Swiss-Prot)
  • Protein family: NadB subfamily (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • repressed in the presence of nicotinic acid (NadR) PubMed
  • Regulatory mechanism:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Alessandra Albertini, University of Pavia, Italy homepage

Your additional remarks

References

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Ilaria Marinoni, Simona Nonnis, Carmine Monteferrante, Peter Heathcote, Elisabeth Härtig, Lars H Böttger, Alfred X Trautwein, Armando Negri, Alessandra M Albertini, Gabriella Tedeschi
Characterization of L-aspartate oxidase and quinolinate synthase from Bacillus subtilis.
FEBS J: 2008, 275(20);5090-107
[PubMed:18959769] [WorldCat.org] [DOI] (I p)

Paola Rossolillo, Ilaria Marinoni, Elisa Galli, Anna Colosimo, Alessandra M Albertini
YrxA is the transcriptional regulator that represses de novo NAD biosynthesis in Bacillus subtilis.
J Bacteriol: 2005, 187(20);7155-60
[PubMed:16199587] [WorldCat.org] [DOI] (P p)

D Sun, P Setlow
Cloning, nucleotide sequence, and regulation of the Bacillus subtilis nadB gene and a nifS-like gene, both of which are essential for NAD biosynthesis.
J Bacteriol: 1993, 175(5);1423-32
[PubMed:8444804] [WorldCat.org] [DOI] (P p)