Tmk

• Description: thymidylate kinase
  
  

Gene name	tmk
Synonyms	yaaP
Essential	yes PubMed
Product	thymidylate kinase
Function	TTP biosynthesis
Metabolic function and regulation of this protein in SubtiPathways: Nucleotides (regulation)
MW, pI	23 kDa, 4.866
Gene length, protein length	636 bp, 212 aa
Immediate neighbours	yaaO, yaaQ
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, essential genes

The gene

Basic information

• Locus tag: BSU00280

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + dTMP = ADP + dTDP (according to Swiss-Prot)

• Protein family: thymidylate kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2CCK (from Staphylococcus aureus, 53% identity, 74% similarity) PubMed

• UniProt: P37537

• KEGG entry: [2]

• E.C. number: 2.7.4.9

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Masayo Kotaka, Balvinder Dhaliwal, Jingshan Ren, Charles E Nichols, Richard Angell, Michael Lockyer, Alastair R Hawkins, David K Stammers
Structures of S. aureus thymidylate kinase reveal an atypical active site configuration and an intermediate conformational state upon substrate binding.
Protein Sci: 2006, 15(4);774-84
[PubMed:16522804] [WorldCat.org] [DOI] (P p)