YvcJ

• Description: nucleotide-binding protein
  
  

Gene name	yvcJ
Synonyms
Essential	no
Product	nucleotide-binding P-loop-containing protein
Function	required for the expression of late competence genes
MW, pI	33 kDa, 6.702
Gene length, protein length	885 bp, 295 aa
Immediate neighbours	yvcK, yvcI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

genetic competence

The gene

Basic information

• Locus tag: BSU34770

Phenotypes of a mutant

reduced competence PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds and hydrolyzes nucleotides, required for the expression of late competence genes (comK, comS) PubMed

• Protein family: UPF0042 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O06973

• KEGG entry: [2]

• E.C. number:

Additional information

• Information on the E. coli homolog

Expression and regulation

• Operon: yvcI-yvcJ-yvcK-yvcL-crh-yvcN PubMed

• Sigma factor: SigA PubMed

• Regulation: very weak stimuation of expression by citrate and succinate PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector: pGP735 (N-terminal Strep-tag, purification from B. subtilis, for SPINE, in pGP380), available in Stülke lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Görke lab

• Antibody:

Labs working on this gene/protein

Anne Galinier, University of Marseille, France

References

Publications on the E. coli homolog

Falk Kalamorz, Birte Reichenbach, Walter März, Bodo Rak, Boris Görke
Feedback control of glucosamine-6-phosphate synthase GlmS expression depends on the small RNA GlmZ and involves the novel protein YhbJ in Escherichia coli.
Mol Microbiol: 2007, 65(6);1518-33
[PubMed:17824929] [WorldCat.org] [DOI] (P p)