KdgA

• Description: 2-dehydro-3-deoxy-phosphogluconate aldolase
  
  

Gene name	kdgA
Synonyms
Essential	no
Product	2-dehydro-3-deoxy-phosphogluconate aldolase
Function	utilization of galacturonic acid
MW, pI	20 kDa, 5.273
Gene length, protein length	588 bp, 196 aa
Immediate neighbours	kdgT, kdgK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon, KdgR regulon

The gene

Basic information

• Locus tag: BSU22100

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 4-hydroxy-2-oxoglutarate = pyruvate + glyoxylate (according to Swiss-Prot)

• Protein family: anti-sigma-factor family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P50846

• KEGG entry: [3]

• E.C. number: 4.1.3.16

Expression and regulation

• Operon: kdgR-kdgK-kdgA-kdgT PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by galacturonate (KdgR) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • KdgR: transcription repression PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Petar Pujic, Rozenn Dervyn, Alexei Sorokin, S Dusko Ehrlich
The kdgRKAT operon of Bacillus subtilis: detection of the transcript and regulation by the kdgR and ccpA genes.
Microbiology (Reading): 1998, 144 ( Pt 11);3111-3118
[PubMed:9846747] [WorldCat.org] [DOI] (P p)