RbsB

• Description: ribose ABC transporter (binding protein)
  
  

Gene name	rbsB
Synonyms
Essential	no
Product	ribose ABC transporter (binding protein)
Function	ribose uptake
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	32 kDa, 6.138
Gene length, protein length	915 bp, 305 aa
Immediate neighbours	rbsC, ywsB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU35960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: bacterial solute-binding protein 2 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: RbsA(2)-(RbsC-RbsD)-RbsB PubMed

• Localization: extracellular (signal peptide), lipid modification as retention signal PubMed, attached to the cell membrane (via RbsC-RbsD) PubMed

Database entries

• Structure: 2GX6 (from Escherichia coli k12 mutant, 54% identity, 71% similarity)

• UniProt: P36949

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rbsR-rbsK-rbsD-rbsA-rbsC-rbsB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by glucose (CcpA) PubMed
  • expressed upon transition into the stationary phase (AbrB) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Birgit Voigt, Haike Antelmann, Dirk Albrecht, Armin Ehrenreich, Karl-Heinz Maurer, Stefan Evers, Gerhard Gottschalk, Jan Maarten van Dijl, Thomas Schweder, Michael Hecker
Cell physiology and protein secretion of Bacillus licheniformis compared to Bacillus subtilis.
J Mol Microbiol Biotechnol: 2009, 16(1-2);53-68
[PubMed:18957862] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Dirk Albrecht, Rabea Sietmann, Frieder Schauer, Michael Hecker, Haike Antelmann
Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis.
Environ Microbiol: 2006, 8(8);1408-27
[PubMed:16872404] [WorldCat.org] [DOI] (P p)

Y Quentin, G Fichant, F Denizot
Inventory, assembly and analysis of Bacillus subtilis ABC transport systems.
J Mol Biol: 1999, 287(3);467-84
[PubMed:10092453] [WorldCat.org] [DOI] (P p)

M A Strauch
AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon.
J Bacteriol: 1995, 177(23);6727-31
[PubMed:7592460] [WorldCat.org] [DOI] (P p)

K Woodson, K M Devine
Analysis of a ribose transport operon from Bacillus subtilis.
Microbiology (Reading): 1994, 140 ( Pt 8);1829-38
[PubMed:7921236] [WorldCat.org] [DOI] (P p)

M O'Reilly, K Woodson, B C Dowds, K M Devine
The citrulline biosynthetic operon, argC-F, and a ribose transport operon, rbs, from Bacillus subtilis are negatively regulated by Spo0A.
Mol Microbiol: 1994, 11(1);87-98
[PubMed:7511775] [WorldCat.org] [DOI] (P p)