MtlR

• Description: transcriptional activator of the mtlA-mtlF-mtlD operon
  
  

Gene name	mtlR
Synonyms	ydaA
Essential	no
Product	transcriptional activator, PRD-type
Function	regulation of mannitol utilization
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	78 kDa, 5.313
Gene length, protein length	2082 bp, 694 aa
Immediate neighbours	ycsN, ydaB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU04160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains: N-terminal DNA-binding domains, two PTS-regulation domains (PRD1 and PRD2), EIIB (Gat)-like domain, EIIA (Mtl)-like domain

• Modification:
  • phosphorylated on His-342 and/or His-399 by PtsH PubMed
  • phosphorylation on His-599 by MtlA PubMed
  • phosphorylation on Cys-419 by MtlF PubMed

• Cofactor(s):

• Effectors of protein activity:
  • activity is stimulated by PtsH-dependent phosphorylation in PRD2 (mechnism of carbon catabolite repression) PubMed
  • activity is inhibited by MtlF-dependent phosphorylation in the EIIB(Gat)-like domain on Cys-419 (this prevents activity in the absence of mannitol and allows induction in presence of mannitol) PubMed

• Interactions: PtsH-MtlR PubMed, MtlA-MtlR PubMed, MtlF-MtlR PubMed

• Localization:

Database entries

• Structure:

• UniProt: P96574

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mtlR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Philippe Joyet, Meriem Derkaoui, Sandrine Poncet, Josef Deutscher
Control of Bacillus subtilis mtl operon expression by complex phosphorylation-dependent regulation of the transcriptional activator MtlR.
Mol Microbiol: 2010, 76(5);1279-94
[PubMed:20444094] [WorldCat.org] [DOI] (I p)

Shouji Watanabe, Miyuki Hamano, Hiroshi Kakeshita, Keigo Bunai, Shigeo Tojo, Hirotake Yamaguchi, Yasutaro Fujita, Sui-Lam Wong, Kunio Yamane
Mannitol-1-phosphate dehydrogenase (MtlD) is required for mannitol and glucitol assimilation in Bacillus subtilis: possible cooperation of mtl and gut operons.
J Bacteriol: 2003, 185(16);4816-24
[PubMed:12897001] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)