Difference between revisions of "Sandbox"
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− | * '''Description:''' RNA polymerase | + | * '''Description:''' RNA polymerase sigma factor SigI <br/><br/> |
{| align="right" border="1" cellpadding="2" | {| align="right" border="1" cellpadding="2" | ||
|- | |- | ||
|style="background:#ABCDEF;" align="center"|'''Gene name''' | |style="background:#ABCDEF;" align="center"|'''Gene name''' | ||
− | |'' | + | |''sigI'' |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || '' | + | |style="background:#ABCDEF;" align="center"| '''Synonyms''' || ''ykoZ '' |
|- | |- | ||
|style="background:#ABCDEF;" align="center"| '''Essential''' || no | |style="background:#ABCDEF;" align="center"| '''Essential''' || no | ||
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase | + | |style="background:#ABCDEF;" align="center"| '''Product''' || RNA polymerase sigma factor SigI |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Function''' || | + | |style="background:#ABCDEF;" align="center"|'''Function''' || control of a class of heat shock genes |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, | + | |style="background:#ABCDEF;" align="center"| '''MW, pI''' || 29 kDa, 8.306 |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || | + | |style="background:#ABCDEF;" align="center"| '''Gene length, protein length''' || 753 bp, 251 aa |
|- | |- | ||
− | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ | + | |style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[ykoY]]'', ''[[rsgI]]'' |
|- | |- | ||
− | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS: | + | |colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+[EMBLCDS:CAB13218]+-newId sequences] <br/> (Barbe ''et al.'', 2009)''' |
|- | |- | ||
− | |colspan="2" | '''Genetic context''' <br/> [[Image: | + | |colspan="2" | '''Genetic context''' <br/> [[Image:sigI_context.gif]] |
<div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div> | ||
|- | |- | ||
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__TOC__ | __TOC__ | ||
− | + | <br/><br/> | |
=The gene= | =The gene= | ||
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=== Basic information === | === Basic information === | ||
− | * '''Locus tag:''' | + | * '''Locus tag:''' BSU13450 |
===Phenotypes of a mutant === | ===Phenotypes of a mutant === | ||
+ | |||
+ | temperature-sensitive growth on agar plates [http://www.ncbi.nlm.nih.gov/sites/entrez/11157964 PubMed] | ||
=== Database entries === | === Database entries === | ||
− | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/ | + | * '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/sigI-rsgI.html] |
− | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+ | + | * '''SubtiList entry:''' [http://genolist.pasteur.fr/SubtiList/genome.cgi?gene_detail+BG13269] |
=== Additional information=== | === Additional information=== | ||
Line 54: | Line 56: | ||
* '''Catalyzed reaction/ biological activity:''' | * '''Catalyzed reaction/ biological activity:''' | ||
− | * '''Protein family:''' | + | * '''Protein family:''' |
* '''Paralogous protein(s):''' | * '''Paralogous protein(s):''' | ||
− | === | + | === Members of the SigI regulon === |
− | ''[[ | + | ''[[gsiB]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/11157964 PubMed], ''[[mreBH]], [[bcrC]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/18156261 PubMed] |
=== Extended information on the protein === | === Extended information on the protein === | ||
Line 74: | Line 76: | ||
* '''Effectors of protein activity:''' | * '''Effectors of protein activity:''' | ||
− | * '''Interactions:''' [[ | + | * '''Interactions:''' [[RsgI]]-[[SigI]], [[RsgI]]-[[SigI]], the interaction inhibits SigI activity [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed] |
− | * '''Localization:''' | + | * '''Localization:''' |
=== Database entries === | === Database entries === | ||
− | * '''Structure:''' | + | * '''Structure:''' |
− | * '''Swiss prot entry:''' | + | * '''Swiss prot entry:''' |
− | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+ | + | * '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU13450] |
* '''E.C. number:''' | * '''E.C. number:''' | ||
Line 92: | Line 94: | ||
=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' '' | + | * '''Operon:''' |
− | ''[[ | + | * '''Operon:''' ''[[sigI]]-[[rsgI]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed] |
− | * ''' | + | * '''Sigma factor:''' [[SigI]] [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed] |
− | |||
− | * '''Regulation:''' | + | * '''Regulation:''' induced by heat [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed] |
− | * '''Regulatory mechanism:''' | + | * '''Regulatory mechanism:''' |
* '''Additional information:''' | * '''Additional information:''' | ||
Line 119: | Line 120: | ||
=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
− | |||
− | |||
=Your additional remarks= | =Your additional remarks= | ||
Line 126: | Line 125: | ||
=References= | =References= | ||
− | <pubmed> | + | <pubmed>17185538 11157964 18156261, </pubmed> |
− | # M | + | # Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y. (2007) Regulatory role of RsgI in ''sigI'' expression in ''Bacillus subtilis''. ''Microbiology'' '''153:''' 92-101. [http://www.ncbi.nlm.nih.gov/sites/entrez/17185538 PubMed] |
+ | # Zuber, U., K. Drzewiecki, and M. Hecker (2001) Putative sigma factor SigI (YkoZ) of ''Bacillus subtilis'' is induced by heat shock ''J. Bacteriol.'' '''183:''' 1472-1475. [http://www.ncbi.nlm.nih.gov/sites/entrez/11157964 PubMed] | ||
+ | # Tseng, C. L., and Shaw, G. C. (2008) Genetic evidence for the actin homolog gene ''mreBH'' and the bacitracin resistance gene ''bcrC'' as targets of the alternative sigma factor SigI of ''Bacillus subtilis''. ''J. Bacteriol.'' '''190:''' 1561-1567. [http://www.ncbi.nlm.nih.gov/sites/entrez/18156261 PubMed] | ||
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] | # Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed] |
Revision as of 13:36, 8 June 2009
- Description: RNA polymerase sigma factor SigI
Gene name | sigI |
Synonyms | ykoZ |
Essential | no |
Product | RNA polymerase sigma factor SigI |
Function | control of a class of heat shock genes |
MW, pI | 29 kDa, 8.306 |
Gene length, protein length | 753 bp, 251 aa |
Immediate neighbours | ykoY, rsgI |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU13450
Phenotypes of a mutant
temperature-sensitive growth on agar plates PubMed
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity:
- Protein family:
- Paralogous protein(s):
Members of the SigI regulon
gsiB PubMed, mreBH, bcrC PubMed
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization:
Database entries
- Structure:
- Swiss prot entry:
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation: induced by heat PubMed
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Chi-Ling Tseng, Gwo-Chyuan Shaw
Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor SigI of Bacillus subtilis.
J Bacteriol: 2008, 190(5);1561-7
[PubMed:18156261]
[WorldCat.org]
[DOI]
(I p)
Kei Asai, Takafumi Ootsuji, Kazue Obata, Takashi Matsumoto, Yasutaro Fujita, Yoshito Sadaie
Regulatory role of RsgI in sigI expression in Bacillus subtilis.
Microbiology (Reading): 2007, 153(Pt 1);92-101
[PubMed:17185538]
[WorldCat.org]
[DOI]
(P p)
U Zuber, K Drzewiecki, M Hecker
Putative sigma factor SigI (YkoZ) of Bacillus subtilis is induced by heat shock.
J Bacteriol: 2001, 183(4);1472-5
[PubMed:11157964]
[WorldCat.org]
[DOI]
(P p)
- Asai K, Ootsuji T, Obata K, Matsumoto T, Fujita Y, Sadaie Y. (2007) Regulatory role of RsgI in sigI expression in Bacillus subtilis. Microbiology 153: 92-101. PubMed
- Zuber, U., K. Drzewiecki, and M. Hecker (2001) Putative sigma factor SigI (YkoZ) of Bacillus subtilis is induced by heat shock J. Bacteriol. 183: 1472-1475. PubMed
- Tseng, C. L., and Shaw, G. C. (2008) Genetic evidence for the actin homolog gene mreBH and the bacitracin resistance gene bcrC as targets of the alternative sigma factor SigI of Bacillus subtilis. J. Bacteriol. 190: 1561-1567. PubMed
- Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed