BofA

• Description: inhibition of the pro-sigma-K processing machinery
  
  

Gene name	bofA
Synonyms
Essential	no
Product	inhibitor of pro-SigK processing
Function	control of SigK activation
MW, pI	8 kDa, 9.32
Gene length, protein length	261 bp, 87 aa
Immediate neighbours	yaaL, rrnA-16S
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: BofA-SpoIVB PubMed

• Localization: forespore outer membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P24282

• KEGG entry: BSU00230

• E.C. number:

Expression and regulation

• Operon: bofA PubMed

• Sigma factor: SigE PubMed

• Regulation: expressed during sporulation PubMed

• Regulatory mechanism: repressed by SpoIIID PubMed

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Simon Cutting, London, UK homepage

References

1. Dong & Cutting (2004) The PDZ domain of the SpoIVB transmembrane signaling protein enables cis-trans interactions involving multiple partners leading to the activation of the pro-sigmaK processing complex in Bacillus subtilis.J. Biol. Chem. 279: 43468-43478. PubMed
2. Ireton & Grossman (1992 ) Interactions among mutations that cause altered timing of gene expression during sporulation in Bacillus subtilis. J. Bacteriol. 174: 3185-3195. PubMed