Difference between revisions of "IolJ"

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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]''
+
* '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
* '''Regulation:''' repressed by glucose ([[CcpA]])
+
* '''Regulation:'''  
 +
** repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]
 +
** induced by inositol ([[IolR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
* '''Regulatory mechanism:''' [[CcpA]]: transcription repression  
+
* '''Regulatory mechanism:'''  
 +
** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed]
 +
** [[IolR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed]
  
 
* '''Additional information:'''
 
* '''Additional information:'''
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
* [[Yasutaro Fujita]], University of Fukuyama, Japan
 +
* [[Ken-ichi Yoshida]], Kobe University, Japan
  
 
=Your additional remarks=
 
=Your additional remarks=

Revision as of 17:31, 1 June 2009

  • Description: 2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction)

Gene name iolJ
Synonyms fbaB, yxdI
Essential no
Product 2-deoxy-5-keto-gluconic acid-6-phosphate aldolase,
formation of dihydroxyacetone phosphate and
malonic semialdehyde (6th reaction)
Function myo-inositol catabolism
MW, pI 31 kDa, 4.854
Gene length, protein length 870 bp, 290 aa
Immediate neighbours yxdJ, iolI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
FbaB context.gif
This image was kindly provided by SubtiList





The gene

Basic information

  • Coordinates:

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-dehydro-2-deoxy-D-gluconate 6-phosphate = glycerone phosphate + malonate semialdehyde (according to Swiss-Prot)
  • Protein family: IolJ subfamily (according to Swiss-Prot)
  • Paralogous protein(s): FbaA

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: inhibited by alpha-keto acids PubMed
  • Interactions:
  • Localization:

Database entries

  • Structure:

Additional information

Expression and regulation

  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

  1. Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y. (2008) myo-Inositol catabolism in Bacillus subtilis. J Biol Chem. Apr 18;283(16): 10415-24. PubMed