Difference between revisions of "IolJ"
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=Expression and regulation= | =Expression and regulation= | ||
− | * '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]'' | + | * '''Operon:''' ''[[iolA]]-[[iolB]]-[[iolC]]-[[iolD]]-[[iolE]]-[[iolF]]-[[iolG]]-[[iolH]]-[[iolJ]]'' [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] |
− | * '''[[Sigma factor]]:''' [[SigA]] | + | * '''[[Sigma factor]]:''' [[SigA]] [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] |
− | * '''Regulation:''' repressed by glucose ([[CcpA]]) | + | * '''Regulation:''' |
+ | ** repressed by glucose ([[CcpA]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed] | ||
+ | ** induced by inositol ([[IolR]]) [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] | ||
− | * '''Regulatory mechanism:''' [[CcpA]]: transcription repression | + | * '''Regulatory mechanism:''' |
+ | ** [[CcpA]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/10666464 PubMed] | ||
+ | ** [[IolR]]: transcription repression [http://www.ncbi.nlm.nih.gov/sites/entrez/9226270 PubMed] | ||
* '''Additional information:''' | * '''Additional information:''' | ||
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=Labs working on this gene/protein= | =Labs working on this gene/protein= | ||
+ | |||
+ | * [[Yasutaro Fujita]], University of Fukuyama, Japan | ||
+ | * [[Ken-ichi Yoshida]], Kobe University, Japan | ||
=Your additional remarks= | =Your additional remarks= |
Revision as of 17:31, 1 June 2009
- Description: 2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction)
Gene name | iolJ |
Synonyms | fbaB, yxdI |
Essential | no |
Product | 2-deoxy-5-keto-gluconic acid-6-phosphate aldolase, formation of dihydroxyacetone phosphate and malonic semialdehyde (6th reaction) |
Function | myo-inositol catabolism |
MW, pI | 31 kDa, 4.854 |
Gene length, protein length | 870 bp, 290 aa |
Immediate neighbours | yxdJ, iolI |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Coordinates:
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: 5-dehydro-2-deoxy-D-gluconate 6-phosphate = glycerone phosphate + malonate semialdehyde (according to Swiss-Prot)
- Protein family: IolJ subfamily (according to Swiss-Prot)
- Paralogous protein(s): FbaA
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity: inhibited by alpha-keto acids PubMed
- Interactions:
- Localization:
Database entries
- Structure:
- Swiss prot entry: P42420
- KEGG entry: BSU39670
- E.C. number: 4.1.2.29
Additional information
Expression and regulation
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
- Yasutaro Fujita, University of Fukuyama, Japan
- Ken-ichi Yoshida, Kobe University, Japan
Your additional remarks
References
- Yoshida K, Yamaguchi M, Morinaga T, Kinehara M, Ikeuchi M, Ashida H, Fujita Y. (2008) myo-Inositol catabolism in Bacillus subtilis. J Biol Chem. Apr 18;283(16): 10415-24. PubMed