SucD

• Description: succinyl-CoA synthetase (alpha subunit)
  
  

Gene name	sucD
Synonyms
Essential	no
Product	succinyl-CoA synthetase (alpha subunit)
Function	TCA cycle
MW, pI	31 kDa, 5.587
Gene length, protein length	900 bp, 300 aa
Immediate neighbours	sucC, smf
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on (Ser-19 OR Thr-20) PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1JKJ (E. coli)

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number: 6.2.1.5

Expression and regulation

• Operon: sucC-SucD

• Sigma factor:

• Regulation: repressed by glucose (2.4-fold) (CcpA) PubMed

• Regulatory mechanism: CcpA: transcription repression

• Additional information:

Biological materials

• Mutant: GP720 (spc), available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149 PubMed# Macek et al. (2007) The serine/ threonine/ tyrosine phosphoproteome of the model bacterium Bacillus subtilis. Mol. Cell. Proteomics 6: 697-707 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed