dprA

168

conveys incoming ssDNA to [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]; facilitates the displacement of both SSBs ([protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]), increases [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] nucleation onto SSB-coated ssDNA, and mediates DNA strand annealing, required to internalize and to recombine ssDNA with homologous resident duplex, crucial for the [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-dependent acquisition of homologous genes from related species by natural transformation

locus: BSU_16110

Molecular weight: 32.78 kDa

pI: 9.76

Protein length: 297 aa

Gene length: 894 bp

function: [category|SW.3.1.5|DNA repair/ recombination]

product: recombination mediator protein

essential: no

synonyms: dprA, smf

List of homologs in different organisms, belongs to COG0758 (Galperin et al., 2021)

Gene

Coordinates: 1,682,580 → 1,683,473

Phenotypes of a mutant

• strongly impaired genetic recombination [Pubmed|23779106]
• ''[gene|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] [gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]'' double mutants have a strongly reduced chromosomal transformation rate [Pubmed|23779106,22373918]
• loss of plasmid transformation [pubmed|30050509]

The protein

Catalyzed reaction/ biological activity

• binds ssDNA, promotes displacement of [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] and [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] from ssDNA [Pubmed|23779106]
• provides [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] access to ssDNA during chromosomal transformation (together with [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO]) [Pubmed|22373918]
• [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA]-ATP in concert with [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] and [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA] catalyzes DNA strand exchange, with [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB] as an accessory factor [Pubmed|25138221]
• the [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]-[protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA] mediator loads [protein|A44D4677FB70BE8F554BF1001A500F817C7DA95F|recA] onto any fragmented linear SPP1 ssDNA [pubmed|31876108]
• anneals [protein|8DE39680CA7663803A107066FBE9AFFCC77DB72A|ssbA]- or [protein|1CDF658441061EA476E27C2E60DEE45C4F45AC15|ssbB]-coated complementary strands of transfecting SPP1 phage DNA, yielding tailed SPP1 duplex intermediates [pubmed|31876108]
• [protein|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA], [protein|80E6F156764FF30300D034EE6FB44F2DB8338AF3|recO] or viral single strand annealing G35P protein, may catalyze the annealing of complete linear phage genomes with redundant regions at the ends of the molecule, alone or with the help of an exonuclease, to produce a circular unit-length duplex viral genome ready to initiate replication [pubmed|31876108]

Protein family

• DprA/Smf family (single member, according to UniProt)

Structure

• [PDB|3UQZ] (aa 90 ... 310, from Streptococcus pneumoniae, 44% identity) [pubmed|22904190]
• [AF|P39813]

[wiki|Localization]

• transiently localizes to the cell pole, and co-localizes with the DNA uptake machinery [Pubmed|17630974]

Expression and Regulation

Operons

• genes: [gene|D72175937B3956C2BC500FC00CEC8EC57A0A76A8|sucC]-[gene|5900CAC1CE367F33B267673A7FC210A3A269B30E|sucD]-[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]-[gene|41199C76DF8CA804B7B8878D61228B9542A96AE4|topA]

  description: [Pubmed|22383849]

  regulation

  • repressed by glucose (2.4-fold) ([protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]) [Pubmed|12850135]
  • expression is heterogeneous [pubmed|29809139]

  regulatory mechanism

  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA]: repression, [Pubmed|12850135], in [regulon|protein:E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|ccpA regulon]

additional information

• expressed under conditions that trigger genetic competence ([protein|08CFA2C72931A75532D4289BC1D18A826DE9F9CA|comK]) [Pubmed|11948146]

Biological materials

Mutant

• BKE16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE16110 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT,  downstream forward: _UP4_TGAATTATCGTTTGACAAAC
• BKK16110 (Δ[gene|FC53216F600994C862F0C0D017C3FA28EB75DCB6|dprA]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK16110 BGSC] and in [wiki|Jörg Stülke]'s lab, [Pubmed|28189581], upstream reverse: _UP1_CAATAGATTGACCTCCTTTT,  downstream forward: _UP4_TGAATTATCGTTTGACAAAC

labs

• [wiki|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

References

Reviews

• 31950915

Original Publications

• 17630974,17803906,12421306,18045469,11948146,22373918,23779106,23440217,25138221,26786319,28618091,22904190,28911099,30050509,31876108,33264457