Replisome

The replisome is a protein complex that is required and sufficient for replication of both leading and lagging strands.

PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed

RecA colocalizes to the replisome in response to endogenous and exogenous DNA damage and in response to damage-independent fork arrest (formation of DNA repair centers), repair center formation depends on RecO and RecR, and is facilitated by RecF and SsbA PubMed

Related pages

• DNA replication

In vitro reconstitution of the replisome

Glenn M Sanders, H Garry Dallmann, Charles S McHenry
Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases.
Mol Cell: 2010, 37(2);273-81
[PubMed:20122408] [WorldCat.org] [DOI] (I p)

Reviews

Kevin S Lang, Houra Merrikh
The Clash of Macromolecular Titans: Replication-Transcription Conflicts in Bacteria.
Annu Rev Microbiol: 2018, 72;71-88
[PubMed:29856930] [WorldCat.org] [DOI] (I p)

Thomas R Beattie, Rodrigo Reyes-Lamothe
A Replisome's journey through the bacterial chromosome.
Front Microbiol: 2015, 6;562
[PubMed:26097470] [WorldCat.org] [DOI] (P e)

Charles S McHenry
DNA replicases from a bacterial perspective.
Annu Rev Biochem: 2011, 80;403-36
[PubMed:21675919] [WorldCat.org] [DOI] (I p)

Patrick M Schaeffer, Madeleine J Headlam, Nicholas E Dixon
Protein--protein interactions in the eubacterial replisome.
IUBMB Life: 2005, 57(1);5-12
[PubMed:16036556] [WorldCat.org] [DOI] (P p)

Other important publications

Masayuki Su'etsugu, Jeff Errington
The replicase sliding clamp dynamically accumulates behind progressing replication forks in Bacillus subtilis cells.
Mol Cell: 2011, 41(6);720-32
[PubMed:21419346] [WorldCat.org] [DOI] (I p)

Houra Merrikh, Cristina Machón, William H Grainger, Alan D Grossman, Panos Soultanas
Co-directional replication-transcription conflicts lead to replication restart.
Nature: 2011, 470(7335);554-7
[PubMed:21350489] [WorldCat.org] [DOI] (I p)