Difference between revisions of "OppA"

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(Biological materials)
(Biological materials)
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** BP67 (spc) available in [[Fabian Commichau]]'s lab
 
** BP67 (spc) available in [[Fabian Commichau]]'s lab
 
** 1S118 ( ''oppA''::''spec''), {{PubMed|11267663}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S118&Search=1S118 BGSC]
 
** 1S118 ( ''oppA''::''spec''), {{PubMed|11267663}}, available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1S118&Search=1S118 BGSC]
** GP2097 (D(''[[oppA]]-[[oppB]]-[[oppC]]-[[oppD]]-[[oppE]]'')::''aphA3''), available in [[Jörg Stülke]]'s lab
+
** GP2097 (D(''[[oppA]]-[[oppB]]-[[oppC]]-[[oppD]]-[[oppF]]'')::''aphA3''), available in [[Jörg Stülke]]'s lab
  
 
* '''Expression vector:'''
 
* '''Expression vector:'''

Revision as of 08:42, 20 July 2015

Gene name oppA
Synonyms spo0KA
Essential no
Product oligopeptide ABC transporter (binding protein)
Function initiation of sporulation, competence development
Gene expression levels in SubtiExpress: oppA
Interactions involving this protein in SubtInteract: OppA
Metabolic function and regulation of this protein in SubtiPathways:
OppA
MW, pI 61 kDa, 5.722
Gene length, protein length 1635 bp, 545 aa
Immediate neighbours trpS, oppB
Sequences Protein DNA DNA_with_flanks
Genetic context
OppA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
OppA expression.png















Categories containing this gene/protein

ABC transporters, utilization of nitrogen sources other than amino acids, genetic competence, phosphorelay, membrane proteins

This gene is a member of the following regulons

CodY regulon, ScoC regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU11430

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • required for the uptake of quorum sensing peptides such as PhrH PubMed
  • Protein family: bacterial solute-binding protein 5 family (according to Swiss-Prot)
  • Paralogous protein(s): DppE

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification: phosphorylation on (Tyr-301 OR Tyr-303) AND Thr-470 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • repressed during exponential growth (ScoC) PubMed
  • Regulatory mechanism:
    • TnrA: transcription activation PubMed
    • ScoC: transcription repression PubMed
    • CodY: transcription repression, no derepression occurs, however, in a codY mutant, due to increased repression by ScoC PubMed
  • Additional information:
    • A ncRNA is predicted between trpS and oppA PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 1937 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 20139 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References