Difference between revisions of "FliG"

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(Expression and regulation)
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=Expression and regulation=
 
=Expression and regulation=
  
* '''Operon:''' ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
+
* '''Operon:''' part of the [[fla-che operon]] (see there for regulation)
 +
** ''[[flgB]]-[[flgC]]-[[fliE]]-[[fliF]]-[[fliG]]-[[fliH]]-[[fliI]]-[[fliJ]]-[[ylxF]]-[[fliK]]-[[flgD]]-[[flgE]]-[[fliL]]-[[fliM]]-[[fliY]]-[[cheY]]-[[fliZ]]-[[fliP]]-[[fliQ]]-[[fliR]]-[[flhB]]-[[flhA]]-[[flhF]]-[[flhG]]-[[cheB]]-[[cheA]]-[[cheW]]-[[cheC]]-[[cheD]]-[[sigD]]-[[swrB]]'' {{PubMed|9657996,8157612,15175317}}
  
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliG_1694119_1695135_1 fliG] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=fliG_1694119_1695135_1 fliG] {{PubMed|22383849}}
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* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
 
* '''[[Sigma factor]]:''' [[SigA]] {{PubMed|9657996}}, [[SigD]] {{PubMed|9657996}}
  
* '''Regulation:'''  
+
* '''Regulation:''' see [[fla-che operon]]
** repressed under conditions that trigger sporulation ([[Spo0A]]) {{PubMed|14651647}}
 
  
* '''Regulatory mechanism:'''  
+
* '''Regulatory mechanism:''' see [[fla-che operon]]
** [[Spo0A]]: transcription repression {{PubMed|14651647}}
 
** [[CodY]]: transcription repression {{PubMed|12730172}}
 
** [[DegU]] (non-phosphorylated): transcription activation {{PubMed|17850253}}
 
** [[DegU]]-P: transcription repression {{PubMed|24386445}}
 
** [[DegU]]-P in complex with [[SwrA]]: transcription activation {{PubMed|24386445}}
 
  
 
* '''Additional information:'''
 
* '''Additional information:'''

Revision as of 14:10, 12 February 2015

  • Description: flagellar basal-body protein, membrane anchor of the basal body, flagellar motor switch protein, physically transduces force from MotA to the rotation of FliF

Gene name fliG
Synonyms flaA15
Essential no
Product flagellar motor switch protein
Function movement and chemotaxis
Gene expression levels in SubtiExpress: fliG
Interactions involving this protein in SubtInteract: FliG
MW, pI 38 kDa, 4.46
Gene length, protein length 1014 bp, 338 aa
Immediate neighbours fliF, fliH
Sequences Protein DNA DNA_with_flanks
Genetic context
FliG context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
FliG expression.png















Categories containing this gene/protein

motility and chemotaxis, membrane proteins

This gene is a member of the following regulons

CodY regulon, DegU regulon, SigD regulon, Spo0A regulon

The gene

Basic information

  • Locus tag: BSU16220

Phenotypes of a mutant

  • mucoid phenotype due to the overproduction of poly-gamma-glutamate PubMed
  • no secretion of FlgM, permanent inhibition of SigD PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • physically transduces force from MotA to the rotation of FliF
  • Protein family: fliG family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: interaction with EpsE prevents energetization of the flagellum by MotA-MotB PubMed

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 250 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References