Difference between revisions of "RelA"

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(Phenotypes of a mutant)
(Phenotypes of a mutant)
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* requirement for valine {{PubMed|24163341}}
 
* requirement for valine {{PubMed|24163341}}
 
* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
 
* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of ''[[guaB]]'' or inactivation of ''[[codY]]'' {{PubMed|24163341}}
 +
* a ''[[relA]] [[sasA]] [[sasB]]'' triple mutant acquires suppressor mutations in ''[[guaA]], [[guaB]], [[gmk]]'' or inactivation of ''[[codY]]'' {{PubMed|24682323,24163341}}
 
* mutation in ''[[relA]]'' results in increased motility and chaining {{PubMed|25331430}}
 
* mutation in ''[[relA]]'' results in increased motility and chaining {{PubMed|25331430}}
 
* ''[[relA]]'' mutation increases flagellin production via elevated [[SigD]] level {{PubMed|25331430}}
 
* ''[[relA]]'' mutation increases flagellin production via elevated [[SigD]] level {{PubMed|25331430}}

Revision as of 17:55, 10 February 2015

Gene name relA
Synonyms
Essential no
Product GTP pyrophosphokinase
Function synthesis and degradation of (p)ppGpp,
triggering the stringent response
Gene expression levels in SubtiExpress: relA
Metabolic function and regulation of this protein in SubtiPathways:
relA
MW, pI 84 kDa, 6.964
Gene length, protein length 2202 bp, 734 aa
Immediate neighbours dtd, apt
Sequences Protein DNA DNA_with_flanks
Genetic context
RelA context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RelA expression.png















Categories containing this gene/protein

metabolism of signalling nucleotides

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU27600

Phenotypes of a mutant

  • requirement for valine PubMed
  • a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
  • a relA sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed
  • mutation in relA results in increased motility and chaining PubMed
  • relA mutation increases flagellin production via elevated SigD level PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: ATP + GTP = AMP + guanosine 3'-diphosphate 5'-triphosphate (according to Swiss-Prot)
  • Protein family: relA/spoT family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 244 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 573 PubMed

Biological materials

  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Mohamed Marahiel, Marburg University, Germany homepage

Jue D Wang, University of Wisconsin–Madison ResearchGate-Profile

Your additional remarks

References