Difference between revisions of "RoxS"

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* '''Additional information:'''
 
* '''Additional information:'''
** the roxS RNA is degraded by the joined activities of [[rny|RNase Y]] (initial endonucleolytic cleavage) and RNase J1 (processive degradation) {{PubMed|25643072}}
+
** the roxS RNA is degraded by the joined activities of [[rny|RNase Y]] (initial endonucleolytic cleavage) and [[rnjA|RNase J1]] (processive degradation) {{PubMed|25643072}}
** the roxS-ppnKB and roxS-sucC RNA duplexes form a cleavage site for RNase III {{PubMed|25643072}}
+
** the ''[[roxS]]''-''[[ppnKB]]'' and ''[[roxS]]-[[sucC]]'' RNA duplexes form a cleavage site for [[rnc|RNase III]] {{PubMed|25643072}}
  
 
=Biological materials =
 
=Biological materials =
  
 
* '''Mutant:'''
 
* '''Mutant:'''
 
+
** available in [[Ciaran Condon]]'s lab {{PubMed|25643072}}
 
* '''Expression vector:'''
 
* '''Expression vector:'''
 
          
 
          

Latest revision as of 12:37, 4 February 2015

  • Description: small RNA, controls expression of ppnKB and sucC-sucD

Gene name roxS
Synonyms rsaE
Essential no
Product trans-acting regulatory RNA
Function control of ppnKB and sucC-sucD expression
Gene length
Immediate neighbours yizD, yjbH
Gene sequence (+200bp)
Genetic context








Categories containing this gene/protein

ncRNA

This gene is a member of the following regulons

ResD regulon

The RoxS regulon

The gene

Basic information

  • Locus tag:

Phenotypes of a mutant

Database entries

  • BsubCyc: no entry
  • DBTBS entry: no entry
  • SubtiList entry: no entry
  • Structure:

Additional information

  • Similar RNA:

The RNA

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: hybridizes to ppnKB and sucD mRNAs to inhibit their translation and to destabilize them PubMed

Extended information on the RNA

  • Kinetic information

Database entries

  • BsubCyc: ?
  • Structure:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:

Biological materials

  • lacZ fusion:


Labs working on this gene/RNA

Ciaran Condon, Paris

Your additional remarks

References

Sylvain Durand, Frédérique Braun, Efthimia Lioliou, Cédric Romilly, Anne-Catherine Helfer, Laurianne Kuhn, Noé Quittot, Pierre Nicolas, Pascale Romby, Ciarán Condon
A nitric oxide regulated small RNA controls expression of genes involved in redox homeostasis in Bacillus subtilis.
PLoS Genet: 2015, 11(2);e1004957
[PubMed:25643072] [WorldCat.org] [DOI] (I e)