ComGA

• Description: late competence protein, traffic ATPase, binds and transports transforming DNA
  
  

Gene name	comGA
Synonyms
Essential	no
Product	traffic ATPase
Function	genetic competence
Gene expression levels in SubtiExpress: comGA
Interactions involving this protein in SubtInteract: ComGA
Metabolic function and regulation of this protein in SubtiPathways: ComGA
MW, pI	40 kDa, 8.893
Gene length, protein length	1068 bp, 356 aa
Immediate neighbours	comGB, corA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

genetic competence, membrane proteins, phosphoproteins

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU24730

Database entries

• BsubCyc: BSU24730

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: binds and transports transforming DNA

• Protein family: GSP E family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-28, Arg-71- and Arg-330 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • ComGA-ComGB PubMed
  • ComGA-ComFA PubMed
  • ComEC-ComGA PubMed
  • Maf-ComGA PubMed

• Localization:
  • peripheral membrane protein PubMed
  • localizes to cell poles in competent cells PubMed
  • localizes to one cell pole PubMed

Database entries

• BsubCyc: BSU24730

• Structure:

• UniProt: P25953

• KEGG entry: [3]

• E.C. number:

Additional information

• ComGA stability depends on Maf PubMed

Expression and regulation

• Operon: comGA-comGB-comGC-comGD-comGE-comGF-comGG-yqzE PubMed

• Expression browser: comGA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by casamino acids PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:
  • GP1640 trpC2 ymdB::spc comG-gfp (spc), available in Jörg Stülke's lab
  • GP1643 comG-gfp (spc) in the NCIB3610 background, available in Jörg Stülke's lab

• two-hybrid system:

• Antibody:

References

Reviews

Inês Chen, David Dubnau
DNA uptake during bacterial transformation.
Nat Rev Microbiol: 2004, 2(3);241-9
[PubMed:15083159] [WorldCat.org] [DOI] (P p)

Original publications

Alexander K W Elsholz, Kürsad Turgay, Stephan Michalik, Bernd Hessling, Katrin Gronau, Dan Oertel, Ulrike Mäder, Jörg Bernhardt, Dörte Becher, Michael Hecker, Ulf Gerth
Global impact of protein arginine phosphorylation on the physiology of Bacillus subtilis.
Proc Natl Acad Sci U S A: 2012, 109(19);7451-6
[PubMed:22517742] [WorldCat.org] [DOI] (I p)

Kenneth Briley, Angella Dorsey-Oresto, Peter Prepiak, Miguel J Dias, Jessica M Mann, David Dubnau
The secretion ATPase ComGA is required for the binding and transport of transforming DNA.
Mol Microbiol: 2011, 81(3);818-30
[PubMed:21707789] [WorldCat.org] [DOI] (I p)

Kenneth Briley, Peter Prepiak, Miguel J Dias, Jeanette Hahn, David Dubnau
Maf acts downstream of ComGA to arrest cell division in competent cells of B. subtilis.
Mol Microbiol: 2011, 81(1);23-39
[PubMed:21564336] [WorldCat.org] [DOI] (I p)

Miriam Kaufenstein, Martin van der Laan, Peter L Graumann
The three-layered DNA uptake machinery at the cell pole in competent Bacillus subtilis cells is a stable complex.
J Bacteriol: 2011, 193(7);1633-42
[PubMed:21278288] [WorldCat.org] [DOI] (I p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Jeanette Hahn, Berenike Maier, Bert Jan Haijema, Michael Sheetz, David Dubnau
Transformation proteins and DNA uptake localize to the cell poles in Bacillus subtilis.
Cell: 2005, 122(1);59-71
[PubMed:16009133] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)

Hanne Jarmer, Randy Berka, Steen Knudsen, Hans H Saxild
Transcriptome analysis documents induced competence of Bacillus subtilis during nitrogen limiting conditions.
FEMS Microbiol Lett: 2002, 206(2);197-200
[PubMed:11814663] [WorldCat.org] [DOI] (P p)

B J Haijema, J Hahn, J Haynes, D Dubnau
A ComGA-dependent checkpoint limits growth during the escape from competence.
Mol Microbiol: 2001, 40(1);52-64
[PubMed:11298275] [WorldCat.org] [DOI] (P p)

Y S Chung, F Breidt, D Dubnau
Cell surface localization and processing of the ComG proteins, required for DNA binding during transformation of Bacillus subtilis.
Mol Microbiol: 1998, 29(3);905-13
[PubMed:9723928] [WorldCat.org] [DOI] (P p)

Y S Chung, D Dubnau
All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis.
J Bacteriol: 1998, 180(1);41-5
[PubMed:9422590] [WorldCat.org] [DOI] (P p)

D van Sinderen, A ten Berge, B J Hayema, L Hamoen, G Venema
Molecular cloning and sequence of comK, a gene required for genetic competence in Bacillus subtilis.
Mol Microbiol: 1994, 11(4);695-703
[PubMed:8196543] [WorldCat.org] [DOI] (P p)

M Albano, R Breitling, D A Dubnau
Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon.
J Bacteriol: 1989, 171(10);5386-404
[PubMed:2507524] [WorldCat.org] [DOI] (P p)