Difference between revisions of "AnsB"

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* '''Additional information:'''
 
* '''Additional information:'''
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 893 {{PubMed|24696501}}
 
** number of protein molecules per cell (complex medium with amino acids, without glucose): 893 {{PubMed|24696501}}
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 118 {{PubMed|21395229}}
 +
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 35 {{PubMed|21395229}}
  
 
=Biological materials =
 
=Biological materials =
 
 
* '''Mutant:''' GP1153 (del ''ansAB''::''ermC'') available in the [[Stülke]] lab
 
* '''Mutant:''' GP1153 (del ''ansAB''::''ermC'') available in the [[Stülke]] lab
  

Revision as of 13:32, 17 April 2014

  • Description: L-aspartase

Gene name ansB
Synonyms
Essential no
Product L-aspartase
Function aspartate degradation
Gene expression levels in SubtiExpress: ansB
Metabolic function and regulation of this protein in SubtiPathways:
ansB
MW, pI 52 kDa, 5.69
Gene length, protein length 1425 bp, 475 aa
Immediate neighbours mleN, ansA
Sequences Protein DNA DNA_with_flanks
Genetic context
AnsB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
AnsB expression.png



















Categories containing this gene/protein

utilization of amino acids, membrane proteins

This gene is a member of the following regulons

AnsR regulon

The gene

Basic information

  • Locus tag: BSU23570

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: L-aspartate = fumarate + NH3 (according to Swiss-Prot)
  • Protein family: Aspartase subfamily (according to Swiss-Prot)
  • Paralogous protein(s): CitG

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 3R6Q (from Bacillus sp. YM55-1, 72% identity, 92% similarity) PubMed
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the presence of asparagine (AnsR) PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 893 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 118 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 35 PubMed

Biological materials

  • Mutant: GP1153 (del ansAB::ermC) available in the Stülke lab
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Additional publications: PubMed