YisP

• Description: production of squalene-derived polyisoprenoids
  
  

Gene name	yisP
Synonyms	yucD
Essential	no
Product	squalene synthase
Function	control of KinC activity
Gene expression levels in SubtiExpress: yisP
MW, pI	31 kDa, 7.258
Gene length, protein length	822 bp, 274 aa
Immediate neighbours	yizA, yisQ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, phosphorelay, biofilm formation

The gene

Basic information

• Locus tag: BSU10810

Phenotypes of a mutant

• complete loss of pellicle-forming ability PubMed
• reduced protein secretion PubMed

Database entries

• BsubCyc: BSU10810

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): NADH PubMed

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU10810

• Structure:

• UniProt: O06728

• KEGG entry: [2]

• E.C. number:

Additional information

The gene is annotated in KEGG as an ortholog of phytoene synthase EC 2.5.1.32. No EC annotation is available in Swiss-Prot/MetaCSwiss-Prot/MetaCyc.literature/experimental evidence supporting the annotation is available. PubMed

Expression and regulation

• Operon:

• Expression browser: yisP PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 37 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Juri Niño Bach, Marc Bramkamp
Flotillins functionally organize the bacterial membrane.
Mol Microbiol: 2013, 88(6);1205-17
[PubMed:23651456] [WorldCat.org] [DOI] (I p)

Yumei Hu, Shiru Jia, Feifei Ren, Chun Hsiang Huang, Tzu Ping Ko, Douglas A Mitchell, Rey Ting Guo, Yingying Zheng
Crystallization and preliminary X-ray diffraction analysis of YisP protein from Bacillus subtilis subsp. subtilis strain 168.
Acta Crystallogr Sect F Struct Biol Cryst Commun: 2013, 69(Pt 1);77-9
[PubMed:23295493] [WorldCat.org] [DOI] (I p)

Daniel López, Roberto Kolter
Functional microdomains in bacterial membranes.
Genes Dev: 2010, 24(17);1893-902
[PubMed:20713508] [WorldCat.org] [DOI] (I p)

Tzu-Lin Hsiao, Olga Revelles, Lifeng Chen, Uwe Sauer, Dennis Vitkup
Automatic policing of biochemical annotations using genomic correlations.
Nat Chem Biol: 2010, 6(1);34-40
[PubMed:19935659] [WorldCat.org] [DOI] (I p)