PyrH

• Description: uridylate kinase
  
  

Gene name	pyrH
Synonyms	smbA
Essential	no
Product	uridylate kinase
Function	pyrimidine biosynthesis
Gene expression levels in SubtiExpress: pyrH
Metabolic function and regulation of this protein in SubtiPathways: pyrH
MW, pI	25 kDa, 5.101
Gene length, protein length	720 bp, 240 aa
Immediate neighbours	tsf, frr
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides

This gene is a member of the following regulons

stringent response

The gene

Basic information

• Locus tag: BSU16510

Database entries

• BsubCyc: BSU16510

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + UMP = ADP + UDP (according to Swiss-Prot)

• Protein family: UMP kinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• BsubCyc: BSU16510

• Structure: 2JJX (from Bacillus anthracis, 42% identity, 64% similarity) PubMed

• UniProt: O31749

• KEGG entry: [2]

• E.C. number: 2.7.4.22

Expression and regulation

• Operon:

• Expression browser: pyrH PubMed

• Sigma factor:

• Regulation:
  • RelA dependent downregulation (Class I) during stringent response PubMed

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 264 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 2015 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Cristina Gagyi, Nadia Bucurenci, Ovidiu Sîrbu, Gilles Labesse, Mihaela Ionescu, Augustin Ofiteru, Liliane Assairi, Stéphanie Landais, Antoine Danchin, Octavian Bârzu, Anne-Marie Gilles
UMP kinase from the Gram-positive bacterium Bacillus subtilis is strongly dependent on GTP for optimal activity.
Eur J Biochem: 2003, 270(15);3196-204
[PubMed:12869195] [WorldCat.org] [DOI] (P p)

R J Turner, Y Lu, R L Switzer
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.
J Bacteriol: 1994, 176(12);3708-22
[PubMed:8206849] [WorldCat.org] [DOI] (P p)

C L Quinn, B T Stephenson, R L Switzer
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.
J Biol Chem: 1991, 266(14);9113-27
[PubMed:1709162] [WorldCat.org] (P p)