Difference between revisions of "PurR"

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* '''Additional information:'''
 
* '''Additional information:'''
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** number of protein molecules per cell (minimal medium with glucose and ammonium): 301 {{PubMed|24696501}}
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** number of protein molecules per cell (complex medium with amino acids, without glucose): 250 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =

Revision as of 09:26, 17 April 2014

  • Description: transcription repressor of the pur operon

Gene name purR
Synonyms yabI
Essential no
Product transcription repressor of the pur operon
Function regulation of purine biosynthesis
Gene expression levels in SubtiExpress: purR
Interactions involving this protein in SubtInteract: PurR
Metabolic function and regulation of this protein in SubtiPathways:
purR
MW, pI 31 kDa, 5.771
Gene length, protein length 855 bp, 285 aa
Immediate neighbours ispE, yabJ
Sequences Protein DNA DNA_with_flanks
Genetic context
PurR context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PurR expression.png




























Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, transcription factors and their control

This gene is a member of the following regulons

PurR regulon

The PurR regulon

The gene

Basic information

  • Locus tag: BSU00470

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Biological activity: transcriptional repressor of purine biosynthetic operons
  • Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity: PRPP acts as inducer, binding results in release of PurR from the operator PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

requires YabJ for in vivo activity

Expression and regulation

  • Sigma factor:
  • Regulation: negative autoregulation, induction in the presence of PRPP PubMed
    • repressed in the presence of purine nucleotides (PurR) PubMed
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 301 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 250 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References