ispE

168

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase, fourth step in the MEP pathway of isoprenoid biosynthesis

Locus: BSU_00460

Molecular weight: 31.57 kDa

Isoelectric point: 6.35

Protein length: 289 aa

Gene length: 870 bp

Function: MEP pathway of isoprenoid biosynthesis

Product: 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Essential: yes

E.C.: 2.7.1.148

Synonyms: ispE, yabH

Genomic Context

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List of homologs in different organisms, belongs to COG1947 (Galperin et al., 2021)

Gene

Coordinates: 53,516  54,385

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol (according to UniProt)
• 4-CDP-2-C-methyl-D-erythritol + ATP --> 4-CDP-2-C-methyl-D-erythritol 2-phosphate + ADP + H⁺ (according to UniProt)

Protein family

• GHMP kinase family (together with GalK and ThrB) (according to UniProt)

Structure

• 1UEK (PDB) (from E. coli, 38% identity) PubMed
• P37550 (AlphaFold)

Expression and Regulation

Operons

• Genes: ispE-purR-yabJ

  Description: PubMed

  Regulation

  • negative autoregulation PubMed

  Regulatory mechanism

  • PurR: repression, PubMed, in purR regulon

Biological materials

Mutant

• MGNA-B910 (yabH::erm), available at the NBRP B. subtilis, Japan

References

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