Difference between revisions of "UvrB"

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(Original publications)
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35170&redirect=T BSU35170]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uvrBA.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/uvrBA.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU35170&redirect=T BSU35170]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2NMV 2NMV] (bound to fluorescein-adducted DNA); [http://www.rcsb.org/pdb/explore.do?structureId=2D7D 2D7D] ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP {{PubMed|16426634}}
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore.do?structureId=2NMV 2NMV] (bound to fluorescein-adducted DNA); [http://www.rcsb.org/pdb/explore.do?structureId=2D7D 2D7D] ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP {{PubMed|16426634}}

Revision as of 14:54, 2 April 2014

  • Description: excinuclease ABC (subunit B)

Gene name uvrB
Synonyms dinA, uvrA
Essential no
Product excinuclease ABC (subunit B)
Function DNA repair after UV damage
Gene expression levels in SubtiExpress: uvrB
Interactions involving this protein in SubtInteract: UvrB
MW, pI 76 kDa, 5.26
Gene length, protein length 1983 bp, 661 aa
Immediate neighbours uvrA, csbA
Sequences Protein DNA DNA_with_flanks
Genetic context
UvrB context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
UvrB expression.png















Categories containing this gene/protein

DNA repair/ recombination

This gene is a member of the following regulons

LexA regulon

The gene

Basic information

  • Locus tag: BSU35170

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

  • A mutation was found in this gene after evolution under relaxed selection for sporulation PubMed


The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: uvrB family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 2NMV (bound to fluorescein-adducted DNA); 2D7D ternary complex involving UvrB* (a C-terminal truncation of full-length UvrB), a polythymine trinucleotide and ADP PubMed
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant: GP1175 (del uvrAB::ermC) (available in the Stülke lab)
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Justin S Lenhart, Jeremy W Schroeder, Brian W Walsh, Lyle A Simmons
DNA repair and genome maintenance in Bacillus subtilis.
Microbiol Mol Biol Rev: 2012, 76(3);530-64
[PubMed:22933559] [WorldCat.org] [DOI] (I p)

James J Truglio, Deborah L Croteau, Bennett Van Houten, Caroline Kisker
Prokaryotic nucleotide excision repair: the UvrABC system.
Chem Rev: 2006, 106(2);233-52
[PubMed:16464004] [WorldCat.org] [DOI] (P p)

Bennett Van Houten, Deborah L Croteau, Matthew J DellaVecchia, Hong Wang, Caroline Kisker
'Close-fitting sleeves': DNA damage recognition by the UvrABC nuclease system.
Mutat Res: 2005, 577(1-2);92-117
[PubMed:15927210] [WorldCat.org] [DOI] (P p)

A Sancar
Mechanisms of DNA excision repair.
Science: 1994, 266(5193);1954-6
[PubMed:7801120] [WorldCat.org] [DOI] (P p)

Original publications