Difference between revisions of "RapI"

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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU05010&redirect=T BSU05010]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rapI-phrI.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/rapI-phrI.html]
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=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU05010&redirect=T BSU05010]
  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4I1A 4I1A] {{PubMed|23526881}}  
 
* '''Structure:''' [http://www.rcsb.org/pdb/explore/explore.do?structureId=4I1A 4I1A] {{PubMed|23526881}}  

Revision as of 13:03, 2 April 2014

Gene name rapI
Synonyms yddL
Essential no
Product response regulator aspartate phosphatase
Function control of transfer of the mobile
genetic element ICEBs1
Gene expression levels in SubtiExpress: rapI
Interactions involving this protein in SubtInteract: RapI
MW, pI 46 kDa, 5.054
Gene length, protein length 1173 bp, 391 aa
Immediate neighbours yddK, phrI
Sequences Protein DNA DNA_with_flanks
Genetic context
RapI context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
RapI expression.png















Categories containing this gene/protein

protein modification, transcription factors and their control, quorum sensing, mobile genetic elements

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU05010

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: binds ImmR and inhibits its activity, this results in induction of the genes for the transfer of the mobile genetic element ICEBs1 PubMed
  • Protein family: RAP family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity: binding of PhrI indicates that neighbour cells do already contain the genetic element ICEBs1, this results in inactivation of RapI PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Vijay Parashar, Philip D Jeffrey, Matthew B Neiditch
Conformational change-induced repeat domain expansion regulates Rap phosphatase quorum-sensing signal receptors.
PLoS Biol: 2013, 11(3);e1001512
[PubMed:23526881] [WorldCat.org] [DOI] (I p)

Jennifer M Auchtung, Catherine A Lee, Katherine L Garrison, Alan D Grossman
Identification and characterization of the immunity repressor (ImmR) that controls the mobile genetic element ICEBs1 of Bacillus subtilis.
Mol Microbiol: 2007, 64(6);1515-28
[PubMed:17511812] [WorldCat.org] [DOI] (P p)

R S McQuade, N Comella, A D Grossman
Control of a family of phosphatase regulatory genes (phr) by the alternate sigma factor sigma-H of Bacillus subtilis.
J Bacteriol: 2001, 183(16);4905-9
[PubMed:11466295] [WorldCat.org] [DOI] (P p)