Difference between revisions of "PlsX"

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===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
** essential {{PubMed|24224907,12682299}}
+
* essential {{PubMed|24224907,12682299}}
** depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
+
* depletion results in sporulation deficiency (via [[SpoIIR]]) {{PubMed|22111992}}
** a ts mutant forms an abberant Z-ring and has a [[cell division]] defect {{PubMed|24224907}}     
+
* a ts mutant forms an abberant Z-ring and has a [[cell division]] defect {{PubMed|24224907}}     
 
 
  
 
=== Database entries ===
 
=== Database entries ===

Revision as of 19:21, 12 January 2014

  • Description: acyl-acyl carrier protein (ACP):phosphate acyltransferase, catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4), coordinates membrane synthesis with cell division

Gene name plsX
Synonyms ylpD
Essential yes PubMed
Product acyl-acyl carrier protein (ACP):phosphate acyltransferase
Function biosynthesis of phospholipids
Gene expression levels in SubtiExpress: plsX
Interactions involving this protein in SubtInteract: PlsX
Metabolic function and regulation of this protein in SubtiPathways:
plsX
MW, pI 35 kDa, 5.492
Gene length, protein length 999 bp, 333 aa
Immediate neighbours fapR, fabD
Sequences Protein DNA DNA_with_flanks
Genetic context
PlsX context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PlsX expression.png















Categories containing this gene/protein

cell division, biosynthesis of lipids, essential genes

This gene is a member of the following regulons

ComA regulon, FapR regulon

The gene

Basic information

  • Locus tag: BSU15890

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: catalyzes the synthesis of the intermediate fatty acyl-phosphate (acyl-PO4)
  • Protein family: plsX family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Modification:
  • Effectors of protein activity:

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced if the cells experience an accumulation of malonyl-CoA (FapR) PubMed
    • expressed at high cell density (ComA) PubMed
    • induced upon fatty acid biosynthesis inhibition PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Matsumoto lab PubMed
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References