CotT

• Description: spore coat protein (inner)
  
  

Gene name	cotT
Synonyms
Essential	no
Product	spore coat protein (inner)
Function	resistance of the spore
Gene expression levels in SubtiExpress: cotT
MW, pI	12 kDa, 9.466
Gene length, protein length	321 bp, 107 aa
Immediate neighbours	ctaO, pdaC
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins

This gene is a member of the following regulons

SigK regulon, GerE regulon, SigE regulon, SpoIIID regulon

The gene

Basic information

• Locus tag: BSU12090

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • inner spore coat, localization depends on SafA PubMed

Database entries

• Structure:

• UniProt: P11863

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cotT PubMed

• Expression browser: cotT PubMed

• Sigma factor: SigK PubMed, SigE PubMed

• Regulation:
  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

• Regulatory mechanism:
  • SpoIIID: transcription activation PubMed
  • GerE: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:
  • 1S108 ( cotT::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Arthur Aronson, Purdue University, West Lafayette, USA homepage

References

Reviews

Peter T McKenney, Adam Driks, Patrick Eichenberger
The Bacillus subtilis endospore: assembly and functions of the multilayered coat.
Nat Rev Microbiol: 2013, 11(1);33-44
[PubMed:23202530] [WorldCat.org] [DOI] (I p)

Original publications

Peter T McKenney, Patrick Eichenberger
Dynamics of spore coat morphogenesis in Bacillus subtilis.
Mol Microbiol: 2012, 83(2);245-60
[PubMed:22171814] [WorldCat.org] [DOI] (I p)

Daisuke Imamura, Ritsuko Kuwana, Hiromu Takamatsu, Kazuhito Watabe
Localization of proteins to different layers and regions of Bacillus subtilis spore coats.
J Bacteriol: 2010, 192(2);518-24
[PubMed:19933362] [WorldCat.org] [DOI] (I p)

Leif Steil, Mónica Serrano, Adriano O Henriques, Uwe Völker
Genome-wide analysis of temporally regulated and compartment-specific gene expression in sporulating cells of Bacillus subtilis.
Microbiology (Reading): 2005, 151(Pt 2);399-420
[PubMed:15699190] [WorldCat.org] [DOI] (P p)

Patrick Eichenberger, Masaya Fujita, Shane T Jensen, Erin M Conlon, David Z Rudner, Stephanie T Wang, Caitlin Ferguson, Koki Haga, Tsutomu Sato, Jun S Liu, Richard Losick
The program of gene transcription for a single differentiating cell type during sporulation in Bacillus subtilis.
PLoS Biol: 2004, 2(10);e328
[PubMed:15383836] [WorldCat.org] [DOI] (I p)

H Takamatsu, T Kodama, A Imamura, K Asai, K Kobayashi, T Nakayama, N Ogasawara, K Watabe
The Bacillus subtilis yabG gene is transcribed by SigK RNA polymerase during sporulation, and yabG mutant spores have altered coat protein composition.
J Bacteriol: 2000, 182(7);1883-8
[PubMed:10714992] [WorldCat.org] [DOI] (P p)

N Bourne, P C FitzJames, A I Aronson
Structural and germination defects of Bacillus subtilis spores with altered contents of a spore coat protein.
J Bacteriol: 1991, 173(20);6618-25
[PubMed:1917883] [WorldCat.org] [DOI] (P p)

A I Aronson, H Y Song, N Bourne
Gene structure and precursor processing of a novel Bacillus subtilis spore coat protein.
Mol Microbiol: 1989, 3(3);437-44
[PubMed:2546006] [WorldCat.org] [DOI] (P p)