SpoIIP

• Description: cell wall hydrolase with both amidase and endopeptidase activities, required for dissolution of the septal cell wall
  
  

Gene name	spoIIP
Synonyms
Essential	no
Product	cell wall hydrolase
Function	dissolution of the septal cell wall
Gene expression levels in SubtiExpress: spoIIP
Interactions involving this protein in SubtInteract: SpoIIP
MW, pI	44 kDa, 6.852
Gene length, protein length	1203 bp, 401 aa
Immediate neighbours	yqxA, gpr
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell wall/ other, sporulation proteins, membrane proteins

This gene is a member of the following regulons

SigE regulon, SigF regulon, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU25530

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • removes the stem peptides from the cell wall and cleaves the cross-links between them PubMed
  • required for proper localization of SpoIIQ (together with SpoIID) PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity: activity is stimulated by interaction with SpoIID PubMed

• Interactions:
  • SpoIID-SpoIIP PubMed
  • SpoIIP-SpoIIM PubMed

• Localization: cell membrane PubMed

Database entries

• Structure:

• UniProt: P37968

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: spoIIP-yqxA PubMed, gpr-spoIIP PubMed

• Expression browser: spoIIP PubMed

• Sigma factor:
  • spoIIP: SigE PubMed
  • gpr:SigF PubMed

• Regulation: expressed early during sporulation (SigE,SigF) PubMed

• Regulatory mechanism:

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Lotte Søgaard-Andersen
Stably bridging a great divide: localization of the SpoIIQ landmark protein in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1019-24
[PubMed:23944268] [WorldCat.org] [DOI] (I p)

Original publications

Jennifer Fredlund, Dan Broder, Tinya Fleming, Clémence Claussin, Kit Pogliano
The SpoIIQ landmark protein has different requirements for septal localization and immobilization.
Mol Microbiol: 2013, 89(6);1053-68
[PubMed:23859254] [WorldCat.org] [DOI] (I p)

Christopher D A Rodrigues, Kathleen A Marquis, Jeffrey Meisner, David Z Rudner
Peptidoglycan hydrolysis is required for assembly and activity of the transenvelope secretion complex during sporulation in Bacillus subtilis.
Mol Microbiol: 2013, 89(6);1039-52
[PubMed:23834622] [WorldCat.org] [DOI] (I p)

Jennifer Gutierrez, Rachelle Smith, Kit Pogliano
SpoIID-mediated peptidoglycan degradation is required throughout engulfment during Bacillus subtilis sporulation.
J Bacteriol: 2010, 192(12);3174-86
[PubMed:20382772] [WorldCat.org] [DOI] (I p)

Cecile Morlot, Tsuyoshi Uehara, Kathleen A Marquis, Thomas G Bernhardt, David Z Rudner
A highly coordinated cell wall degradation machine governs spore morphogenesis in Bacillus subtilis.
Genes Dev: 2010, 24(4);411-22
[PubMed:20159959] [WorldCat.org] [DOI] (I p)

Arnaud Chastanet, Richard Losick
Engulfment during sporulation in Bacillus subtilis is governed by a multi-protein complex containing tandemly acting autolysins.
Mol Microbiol: 2007, 64(1);139-52
[PubMed:17376078] [WorldCat.org] [DOI] (P p)

Stephanie T Wang, Barbara Setlow, Erin M Conlon, Jessica L Lyon, Daisuke Imamura, Tsutomu Sato, Peter Setlow, Richard Losick, Patrick Eichenberger
The forespore line of gene expression in Bacillus subtilis.
J Mol Biol: 2006, 358(1);16-37
[PubMed:16497325] [WorldCat.org] [DOI] (P p)

Jonathan Dworkin, Richard Losick
Developmental commitment in a bacterium.
Cell: 2005, 121(3);401-9
[PubMed:15882622] [WorldCat.org] [DOI] (P p)

Thierry Doan, Kathleen A Marquis, David Z Rudner
Subcellular localization of a sporulation membrane protein is achieved through a network of interactions along and across the septum.
Mol Microbiol: 2005, 55(6);1767-81
[PubMed:15752199] [WorldCat.org] [DOI] (P p)

P Eichenberger, P Fawcett, R Losick
A three-protein inhibitor of polar septation during sporulation in Bacillus subtilis.
Mol Microbiol: 2001, 42(5);1147-62
[PubMed:11886548] [WorldCat.org] [DOI] (P p)