RapH

• Description: response regulator aspartate phosphatase, dephosphorylates Spo0F-P, control of the phosphorelay, control of ComA activity
  
  

Gene name	rapH
Synonyms	yeeH, yzqA
Essential	no
Product	response regulator aspartate phosphatase
Function	control of sporulation initiation and ComA activity
Gene expression levels in SubtiExpress: rapH
Interactions involving this protein in SubtInteract: RapH
Function and regulation of this protein in SubtiPathways: Phosphorelay
MW, pI	49 kDa, 6.121
Gene length, protein length	1293 bp, 431 aa
Immediate neighbours	yeeG, phrH
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

protein modification, transcription factors and their control, phosphorelay, quorum sensing

This gene is a member of the following regulons

AbrB regulon, ComK regulon, RghR regulon

The gene

Basic information

• Locus tag: BSU06830

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • binds to and thereby inhibits ComA activity PubMed
  • RapH dephosphorylates Spo0F-P and thereby controls the phosphorelay PubMed
  • in addition to dephosphorylating Spo0F, RapH can antagonize sporulation by sterically blocking phosphoryl transfer to and from Spo0F PubMed

• Protein family: RAP family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:
  • interaction with PhrH inhibits dephosphorylation of Spo0F and sequestration of ComA by RapH PubMed

• Interactions:
  • RapH-ComA PubMed
  • RapH-Spo0F-P PubMed
  • RapH-PhrH PubMed

• Localization:

Database entries

• Structure: 3Q15 (RapH-Spo0F complex) PubMed

• UniProt: P40771

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: rapH-phrH PubMed

• Expression browser: rapH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by RghR PubMed

• Regulatory mechanism:
  • RghR: transcription repression PubMed
  • ComK: transcription activation PubMed
  • AbrB: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Nicolas Mirouze, Vijay Parashar, Melinda D Baker, David A Dubnau, Matthew B Neiditch
An atypical Phr peptide regulates the developmental switch protein RapH.
J Bacteriol: 2011, 193(22);6197-206
[PubMed:21908671] [WorldCat.org] [DOI] (I p)

Vijay Parashar, Nicolas Mirouze, David A Dubnau, Matthew B Neiditch
Structural basis of response regulator dephosphorylation by Rap phosphatases.
PLoS Biol: 2011, 9(2);e1000589
[PubMed:21346797] [WorldCat.org] [DOI] (I e)

Onuma Chumsakul, Hiroki Takahashi, Taku Oshima, Takahiro Hishimoto, Shigehiko Kanaya, Naotake Ogasawara, Shu Ishikawa
Genome-wide binding profiles of the Bacillus subtilis transition state regulator AbrB and its homolog Abh reveals their interactive role in transcriptional regulation.
Nucleic Acids Res: 2011, 39(2);414-28
[PubMed:20817675] [WorldCat.org] [DOI] (I p)

Wiep Klaas Smits, Cristina Bongiorni, Jan-Willem Veening, Leendert W Hamoen, Oscar P Kuipers, Marta Perego
Temporal separation of distinct differentiation pathways by a dual specificity Rap-Phr system in Bacillus subtilis.
Mol Microbiol: 2007, 65(1);103-20
[PubMed:17581123] [WorldCat.org] [DOI] (P p)

Kentaro Hayashi, Tsukahara Kensuke, Kazuo Kobayashi, Naotake Ogasawara, Mitsuo Ogura
Bacillus subtilis RghR (YvaN) represses rapG and rapH, which encode inhibitors of expression of the srfA operon.
Mol Microbiol: 2006, 59(6);1714-29
[PubMed:16553878] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Randy M Berka, Jeanette Hahn, Mark Albano, Irena Draskovic, Marjan Persuh, Xianju Cui, Alan Sloma, William Widner, David Dubnau
Microarray analysis of the Bacillus subtilis K-state: genome-wide expression changes dependent on ComK.
Mol Microbiol: 2002, 43(5);1331-45
[PubMed:11918817] [WorldCat.org] [DOI] (P p)