Difference between revisions of "SirA"

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* '''Description:''' sporulation protein, inhibits DNA replication <br/><br/>
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* '''Description:''' [[sporulation]] protein, inhibits [[DNA replication]] <br/><br/>
  
 
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* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yneE_1922017_1922463_1 sirA] {{PubMed|22383849}}
 
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=yneE_1922017_1922463_1 sirA] {{PubMed|22383849}}
  
* '''Sigma factor:'''  
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* '''[[Sigma factor]]:'''  
  
 
* '''Regulation:'''  
 
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=References=
 
=References=
 
==Reviews==
 
==Reviews==
<pubmed> 20157337 </pubmed>
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<pubmed> 20157337 22933559</pubmed>
==Original publications==
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== Original publications ==
 
<pubmed> 19682252, 21239581,14651647, 19329632 9068642</pubmed>
 
<pubmed> 19682252, 21239581,14651647, 19329632 9068642</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 17:32, 13 September 2013

Gene name sirA
Synonyms yneE, yoxF
Essential no
Product inhibitor of DNA replication
Function control of chromosome copy number
Gene expression levels in SubtiExpress: sirA
Interactions involving this protein in SubtInteract: SirA
MW, pI 17 kDa, 7.353
Gene length, protein length 444 bp, 148 aa
Immediate neighbours tkt, yneF
Sequences Protein DNA DNA_with_flanks
Genetic context
YneE context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
SirA expression.png















Categories containing this gene/protein

DNA replication

This gene is a member of the following regulons

Spo0A regulon

The gene

yneE was renamed to sirA.

Basic information

  • Locus tag: BSU17900

Phenotypes of a mutant

"over-replication" during sporulation PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: interacts with DnaA and displaces it from the replication origin, resulting in inhibition of replication PubMed
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • expressed under conditions that trigger sporulation (Spo0A) PubMed
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

sirA (sporulation inhibitor of replication) is activated by Spo0A~P and inhibits DNA replication during sporulation.

References

Reviews

Original publications

Lilah Rahn-Lee, Houra Merrikh, Alan D Grossman, Richard Losick
The sporulation protein SirA inhibits the binding of DnaA to the origin of replication by contacting a patch of clustered amino acids.
J Bacteriol: 2011, 193(6);1302-7
[PubMed:21239581] [WorldCat.org] [DOI] (I p)

Jennifer K Wagner, Kathleen A Marquis, David Z Rudner
SirA enforces diploidy by inhibiting the replication initiator DnaA during spore formation in Bacillus subtilis.
Mol Microbiol: 2009, 73(5);963-74
[PubMed:19682252] [WorldCat.org] [DOI] (I p)

Lilah Rahn-Lee, Boris Gorbatyuk, Ole Skovgaard, Richard Losick
The conserved sporulation protein YneE inhibits DNA replication in Bacillus subtilis.
J Bacteriol: 2009, 191(11);3736-9
[PubMed:19329632] [WorldCat.org] [DOI] (I p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

T Schiött, C von Wachenfeldt, L Hederstedt
Identification and characterization of the ccdA gene, required for cytochrome c synthesis in Bacillus subtilis.
J Bacteriol: 1997, 179(6);1962-73
[PubMed:9068642] [WorldCat.org] [DOI] (P p)