GmuC

• Description: glucomannan-specific phosphotransferase system, EIIC component of the PTS
  
  

Gene name	gmuC
Synonyms	ydhO
Essential	no
Product	glucomannan-specific phosphotransferase system, EIIC component
Function	glucomannan uptake and phosphorylation
Gene expression levels in SubtiExpress: gmuC
Interactions involving this protein in SubtInteract: GmuC
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	48 kDa, 9.803
Gene length, protein length	1326 bp, 442 aa
Immediate neighbours	gmuA, gmuD
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

phosphotransferase systems, utilization of specific carbon sources

This gene is a member of the following regulons

AbrB regulon, CcpA regulon, GmuR regulon, Efp-dependent proteins

The gene

Basic information

• Locus tag: BSU05830

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: PTS permease, lactose permease (Lac) family PubMed

• Paralogous protein(s): LicC, YwbA

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • GmuB-GmuC

• Localization:

Database entries

• Structure: 3QNQ (EIIC component of diacetylchitobiose specific PTS from Bacillus cereus; 41% identity, 80% similarity) PubMed

• UniProt: O05507

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: gmuB-gmuA-gmuC-gmuD-gmuR-gmuE-gmuF-gmuG PubMed

• Expression browser: gmuC PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by conjac glucomannan (requires GmuG) (GmuR) PubMed
  • induced by cellobiose (GmuR) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed

• Regulatory mechanism:
  • GmuR: transcription repression PubMed
  • CcpA: transcription repression PubMed
  • AbrB: transcription activation PubMed

• Additional information:
  • translation is likely to require Efp due to the presence of several consecutive proline residues PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Yoshito Sadaie, Hisashi Nakadate, Reiko Fukui, Lii Mien Yee, Kei Asai
Glucomannan utilization operon of Bacillus subtilis.
FEMS Microbiol Lett: 2008, 279(1);103-9
[PubMed:18177310] [WorldCat.org] [DOI] (P p)

Jonathan Reizer, Steffi Bachem, Aiala Reizer, Maryvonne Arnaud, Milton H Saier, Jörg Stülke
Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis.
Microbiology (Reading): 1999, 145 ( Pt 12);3419-3429
[PubMed:10627040] [WorldCat.org] [DOI] (P p)